FUK Antibody (6E2)

Western Blot: FUK Antibody (6E2) [NBP2-37604] - Western blot analysis using FUK mouse mAb against Hela (1), HepG2 (2), Jurkat (3), A431 (4), HEK293 (5), MCF-7 (6), PC-12 (7), Cos7 (8), and NIH/3T3 (9) cell lysate.

Flow Cytometry: FUK Antibody (6E2) [NBP2-37604] - Flow cytometric analysis of Hela cells using FUK mouse mAb (green) and negative control (purple).

ELISA: FUK Antibody (6E2) [NBP2-37604] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

FUK Antibody (6E2) Summary

• Immunogen: Purified recombinant fragment of human FUK expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUK
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 117 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for FUK Antibody (6E2)

• EC 2.7.1.52
• FLJ39408,1110046B12Rik
• fucokinase
• L-fucose kinase

Background

FUK is encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUK