Fibrinopeptide A Antibody (1D12) - Azide and BSA Free

Western Blot: Fibrinopeptide A Antibody (1D12) [H00002243-M01] - Analysis of FGA expression in transfected 293T cell line by FGA monoclonal antibody (M01), clone 1D12.Lane 1: FGA transfected lysate(24.7 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: Fibrinopeptide A Antibody (1D12) [H00002243-M01] - Analysis of protein-protein interactions between APOA1 and FGA. HeLa cells were stained with anti-APOA1 rabbit purified polyclonal 1:1200 and anti-FGA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Immunoprecipitation: Fibrinopeptide A Antibody (1D12) [H00002243-M01] - Analysis of FGA transfected lysate using anti-FGA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FGA MaxPab rabbit polyclonal antibody.

Sandwich ELISA: Fibrinopeptide A Antibody (1D12) [H00002243-M01] - Detection limit for recombinant GST tagged FGA is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func, IP
• Clone: 1D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Fibrinopeptide A Antibody (1D12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FGA (AAH20764, 1 a.a. ~ 218 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFSMSIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS
• Specificity: FGA - fibrinogen alpha chain (1D12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fibrinopeptide A Antibody (1D12) - Azide and BSA Free

• FGA
• Fib2
• Fibrinogen A
• Fibrinogen alpha chain
• Fibrinogen alpha subunit
• Fibrinogen Type I
• fibrinogen, A alpha polypeptide
• FPA
• MGC119422
• MGC119423
• MGC119425

Background

The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGA
• Entrez:
  • 2243(Human)
• Uniprot:
  • AAH20764(Human)