FAT2 Antibody (8C5)

Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).

Immunohistochemistry-Paraffin: FAT2 Antibody (8C5) [NBP2-53235] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Protocadherin FAT2 Monoclonal Antibody (8C5).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC
• Clone: 8C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

FAT2 Antibody (8C5) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54371)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Purified recombinant human FAT2 protein. (Uniprot: Q9NYQ8)
• Localization: Cell Surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FAT2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 480 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for FAT2 Antibody (8C5)

• protocadherin Fat 2
• CDHF8
• CDHR9
• FAT tumor suppressor homolog 2 (Drosophila)
• HFAT2
• MEGF1

Background

The FAT2 gene is the second identified human homolog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAT2
• Uniprot:
  • Q9NYQ8(Human)