Ext2 Antibody

Western Blot: Ext2 Antibody [NBP2-29948] - Western blot analysis of lysate from T47D cell line,using EXT2 Antibody (Center)(NBP2-29948).AP9401c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.

Immunocytochemistry/Immunofluorescence: Ext2 Antibody [NBP2-29948] - Confocal immunofluorescent analysis of EXT2 Antibody (Center)(NBP2-29948) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry-Paraffin: Ext2 Antibody [NBP2-29948] - Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow Cytometry: Ext2 Antibody [NBP2-29948] - Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Ext2 Antibody Summary

• Immunogen: This EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-209 amino acids from the Central region of human EXT2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EXT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Flow Cytometry 1:10 - 1:50
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
• Theoretical MW: 82.255 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Human. Predicted to react with Bovine and Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Protein A purified

Alternate Names for Ext2 Antibody

• EC 2.4.1.224
• EC 2.4.1.225
• exostoses (multiple) 2
• Exostosin 2
• exostosin-2
• EXT2
• Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan4-alpha-N-acetylglucosaminyltransferase
• Multiple Exostoses Protein 2
• N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase
• Putative tumor suppressor protein EXT2
• SOTV

Background

EXT2 encodes one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EXT2
• Entrez:
  • 2132(Human)
  • 14043(Mouse)
  • 311215(Rat)
• Uniprot:
  • Q93063(Human)