Ext2 Antibody

Images

 
Western Blot: Ext2 Antibody [NBP2-29948] - Western blot analysis of lysate from T47D cell line,using EXT2 Antibody (Center)(NBP2-29948).AP9401c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at ...read more
Immunocytochemistry/ Immunofluorescence: Ext2 Antibody [NBP2-29948] - Confocal immunofluorescent analysis of EXT2 Antibody (Center)(NBP2-29948) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG ...read more
Immunohistochemistry-Paraffin: Ext2 Antibody [NBP2-29948] - Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, ...read more
Flow Cytometry: Ext2 Antibody [NBP2-29948] - Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

Ext2 Antibody Summary

Immunogen
This EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-209 amino acids from the Central region of human EXT2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXT2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10 - 1:50
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry-Paraffin 1:10 - 1:50
Theoretical MW
82.255 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Human. Predicted to react with Bovine and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
0.3 mg/ml
Purity
Protein A purified

Alternate Names for Ext2 Antibody

  • EC 2.4.1.224
  • EC 2.4.1.225
  • exostoses (multiple) 2
  • Exostosin 2
  • exostosin-2
  • EXT2
  • Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan4-alpha-N-acetylglucosaminyltransferase
  • Multiple Exostoses Protein 2
  • N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase
  • Putative tumor suppressor protein EXT2
  • SOTV

Background

EXT2 encodes one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ext2 Antibody (NBP2-29948) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EXT2
Entrez
Uniprot