Home » ErbB3/Her3 » ErbB3/Her3 ELISA Kits » Human ErbB3/Her3 ELISA Kit

Human ErbB3/Her3 ELISA Kit

 Submit a Review

Images

 
(
Enlarge)
ELISA: ErbB3/Her3 ELISA Kit [Biotin] [KA1710] - A standard curve should be generated each time the assay is performed. Column A represents Assay Diluent A and column B represents Assay Diluent B.
ELISA: ErbB3/Her3 ELISA Kit [Biotin] [KA1710] - A standard curve should be generated each time the assay is performed. Column A represents Assay Diluent A and column B represents Assay Diluent B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum, Urine
Sensitivity
4 pg/mL

Order Details

100% Guarantee on every product

Human ErbB3/Her3 ELISA Kit Summary

Sensitivity
4 pg/mL
Inter-Assay
<12%
Intra-Assay
<10%
Kit Type
ELISA Kit

Applications/Dilutions

Application Notes
ERBB3 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ERBB3.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Notes

Detection Method:Colorimetric
Intra-Assay:<10%
Inter-Assay:<12%
Limit of Detection:4 pg/mLThis product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human ErbB3/Her3 ELISA Kit

  • c-erbB3
  • EC 2.7.10
  • EC 2.7.10.1
  • ErbB3
  • ErbB-3
  • erbB3-S
  • HER3
  • HER3c-erbB-3
  • LCCS2
  • lethal congenital contracture syndrome 2
  • MDA-BF-1
  • MGC88033
  • p180-ErbB3
  • p45-sErbB3
  • p85-sErbB3
  • Proto-oncogene-like protein c-ErbB-3
  • receptor tyrosine-protein kinase erbB-3
  • Tyrosine kinase-type cell surface receptor HER3
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

EGFR Antibody
[Unconjugated]
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). A specific band was detected for EGFR at approximately 175&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog&amp;nbsp;#&amp;nbsp;AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# F0107). View our protocol for Staining Membrane-associated Proteins.
EGFR was detected in immersion fixed&amp;nbsp;frozen sections of human&amp;nbsp;skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1&amp;nbsp;µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte&amp;#8482; HRP Polymer Antibody (Catalog&amp;nbsp;# VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(7 Publications)
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
ErbB2/Her2
[Unconjugated]
1129-ER
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing&amp;nbsp;major bands at&amp;nbsp;125-130 kDa and&amp;nbsp; 220-250 kDa, respectively.
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER) blocks anti-ErbB2 mediated inhibition of the SK‑BR‑3 human breast cancer cell line. The ED50 is 20-80 ng/mL in the presence of 0.6 μg/mL Goat Anti-Human ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF1129)
(5 Reviews)
(26 Publications)
Species: Hu
ErbB4/Her4
Antibody (H4.77.16 (A
NB120-3104
Western Blot: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of 25 ug of mouse brain cell lysates.
Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in T47D cells (green) compared to an isotype control (blue).
Flow Cytometry: ErbB4/Her4 Antibody (H4.77.16) [NB120-3104] - Analysis of HER-4 in MCF-7 cells (green) compared to an isotype control (blue).
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IP
EGF [Unconjugated]
236-EG
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
1 µg/lane of Recombinant Human EGF was resolved&amp;nbsp;with SDS-PAGE&amp;nbsp; and visualized&amp;nbsp;by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
(10 Reviews)
(300 Publications)
Species: Hu
Neuregulin-1 beta
1/NRG1 beta 1
396-HB
Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain (Catalog # 396-HB) stimulates cell proliferation of the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.06-0.3 ng/mL.
1 μg/lane of Recombinant Human NRG1-beta 1/HRG1-beta 1 EGF Domain was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.
(36 Publications)
Species: Hu
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100&amp;nbsp;ng/mL Human&amp;nbsp;PDGF&amp;nbsp;(Catalog&amp;nbsp;# 120‑HD) for 15&amp;nbsp;minutes and MCF‑7 human breast cancer cell line untreated or treated with 100&amp;nbsp;ng/mL&amp;nbsp;Recombinant Human IGF-1 (Catalog&amp;nbsp;# 291‑G1) for 15&amp;nbsp;minutes. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group&amp;nbsp;1.
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887) at 10&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog&amp;nbsp;# 291-G1) for 15&amp;nbsp;minutes, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887). This experiment was conducted&amp;nbsp;under&amp;nbsp;reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.
(18 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
PIK3CA Antibody
(OTI6D9)
NBP2-46403
Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Species: Hu
Applications: IHC
NEURL Antibody
NBP1-92177
Immunohistochemistry-Paraffin: NEURL Antibody [NBP1-92177] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
Species: Hu
Applications: IHC, IHC-P
NEU-1/Sialidase-1
Antibody (688215)
MAB6860
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2&amp;nbsp;µg/mL of Mouse Anti-Human NEU‑1/Sialidase‑1 Monoclonal Antibody (Catalog&amp;nbsp;# MAB6860) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog&amp;nbsp;# HAF007). A specific band was detected for NEU‑1/Sialidase‑1 at approximately 45&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, IP
PI 3-Kinase p110
beta/PIK3CB Antibody
NBP1-33116
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - A. 30 ug PC-12 whole cell lysate/extract 5 % SDS-PAGE PI3K p110 beta antibody [N3C2], Internal dilution: 1:1000
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Paraffin-embedded rat testis. PI3K p110 beta antibody [N3C2], Internal diluted at 1:400.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PI 3-Kinase p110
delta Antibody
NBP2-38535
Western Blot: PI 3-Kinase p110 delta Antibody [NBP2-38535] - Lane 1: Marker [kDa] 250,130,100,70,55,35,25,15,10. Lane 2: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 delta Antibody [NBP2-38535] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytokinetic bridge &amp; vesicles.
Immunohistochemistry-Paraffin: PI 3-Kinase p110 delta Antibody [NBP2-38535] - Staining in human lymph node and colon tissues using anti-PIK3CD antibody. Corresponding PIK3CD RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
PI 3-Kinase p110
gamma Antibody
NBP2-15071
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Paraffin-embedded Human Breast Cancer
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Whole cell extract (30ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
HPRG Antibody
[Unconjugated]
AF1905
HPRG Antibody [Unconjugated]
(1 Publication)
Species: Mu
Applications: WB
ERK1 Antibody (1E5)
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for ErbB3/Her3 ELISA Kit (KA1710) (0)

There are no publications for ErbB3/Her3 ELISA Kit (KA1710).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ErbB3/Her3 ELISA Kit (KA1710) (0)

There are no reviews for ErbB3/Her3 ELISA Kit (KA1710). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ErbB3/Her3 ELISA Kit (KA1710) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ErbB3/Her3 Products

Diseases for ErbB3/Her3 ELISA Kit (KA1710)

Discover more about diseases related to ErbB3/Her3 ELISA Kit (KA1710).
 

Pathways for ErbB3/Her3 ELISA Kit (KA1710)

View related products by pathway.

PTMs for ErbB3/Her3 ELISA Kit (KA1710)

Learn more about PTMs related to ErbB3/Her3 ELISA Kit (KA1710).

Blogs on ErbB3/Her3

There are no specific blogs for ErbB3/Her3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SDS-Page: Recombinant Human ErbB3/Her3 Protein [NBP2-52128]
Recombinant Human ErbB3/Her3 Protein
NBP2-52128-0.05mg
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
EGF [Unconjugated]
236-EG-200
Recombinant Human NRG1‑1 beta 1/HRG1‑1 beta 1 (Catalog&nbsp;# 377-HB) stimulates proliferation in the MCF‑17 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1‑1 beta 1/HRG1‑1 beta 1 (10&nbsp;ng/mL) is neutralized (green line) by increasing concentrations of Human ErbB3/Her3 Monoclonal Antibody (Catalog&nbsp;# MAB3481). The ND50 is typically 0.0075-0.03&nbsp;µg/mL.
ErbB3/Her3 Antibody (66223)
MAB3481-100
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
ERK1 Antibody (1E5)
NBP2-22203

Contact Information

Product PDFs

  ErbB3/Her3 ELISA Kit
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human ErbB3/Her3 ELISA Kit and receive a gift card or discount.


Bioinformatics

Entrez