![Western Blot: C9orf11 Antibody [NBP1-90869] - Analysis in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/C9orf11-Antibody-Western-Blot-NBP1-90869-img0006.jpg "Western Blot: C9orf11 Antibody [NBP1-90869] - Analysis in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit EQTN Antibody - BSA Free (NBP1-90869) is a polyclonal antibody validated for use in IHC and WB. Anti-EQTN Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SCESQYSVNPELATMSYFHPSEGVSDTSFSKSAESSTFLGTTSSDMRRSGTRTSESKIMTDIISIGSDNEMHENDESVTR |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EQTN |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-90869 | Applications | Species |
|---|---|---|
| Fagerberg L, Oksvold P, Skogs M et al. Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP). J Proteome Res 2013-06-07 [PMID: 23276153] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EQTN |
![Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/C9orf11-Antibody-Immunohistochemistry-Paraffin-NBP1-90869-img0012.jpg "Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon, liver, prostate and testis using Anti-EQTN antibody NBP1-90869 (A) shows similar protein distribution across tissues to independent antibody NBP1-90868 (B).](http://images.novusbio.com/fullsize/C9orf11-Antibody-Immunohistochemistry-Paraffin-NBP1-90869-img0007.jpg "Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon, liver, prostate and testis using Anti-EQTN antibody NBP1-90869 (A) shows similar protein distribution across tissues to independent antibody NBP1-90868 (B).")
![Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Analysis in human testis and prostate tissues. Corresponding EQTN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/C9orf11-Antibody-Immunohistochemistry-Paraffin-NBP1-90869-img0008.jpg "Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Analysis in human testis and prostate tissues. Corresponding EQTN RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human testis shows strong positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/C9orf11-Antibody-Immunohistochemistry-Paraffin-NBP1-90869-img0009.jpg "Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human testis shows strong positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/C9orf11-Antibody-Immunohistochemistry-Paraffin-NBP1-90869-img0010.jpg "Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human prostate shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/C9orf11-Antibody-Immunohistochemistry-Paraffin-NBP1-90869-img0011.jpg "Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")