EQTN Antibody - BSA Free

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Western Blot: C9orf11 Antibody [NBP1-90868] - Analysis in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90868] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry: C9orf11 Antibody [NBP1-90868] - Staining of human testis shows strong cytoplasmic positivity in a subset of seminiferus duct cells.
Independent Antibodies: Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90868] - Staining of human colon, liver, prostate and testis using Anti-EQTN antibody NBP1-90868 (A) shows similar protein ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90868] - Analysis in human testis and prostate tissues. Corresponding EQTN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90868] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90868] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90868] - Staining of human colon shows no positivity in glandular cells as expected.
Orthogonal Strategies: Analysis in human testis and prostate tissues using NBP1-90868 antibody. Corresponding EQTN RNA-seq data are presented for the same tissues.
Analysis in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

EQTN Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EDVNKQEEKNEDHTPNYAPANEKNGNYYKDIKQYVFTTQNPNGTESEISVRATTDLNFALKNGSTPNVPAFWTMLAKAINGTAVVMDDKDQLFHPIPESDVNATQGENQPDLEDLKIK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EQTN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
EQTN Recombinant Protein Antigen (NBP1-90868PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EQTN Antibody - BSA Free

  • acrosome formation associated factor
  • acrosome formation-associated factor
  • AFAF
  • C9orf11
  • chromosome 9 open reading frame 11
  • equatorin
  • equatorin, sperm acrosome associated
  • SPACA8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EQTN