EMR3 Antibody

Immunohistochemistry-Paraffin: EMR3 Antibody [NBP1-02665] - Staining of human macrophage. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Product Discontinued

EMR3 Antibody Summary

• Immunogen: Synthetic 18 amino acid peptide from C-terminal cytoplasmic domain of human EMR3.
• Specificity: Human EMR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADGRE3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
• Application Notes: IHC: NBP1-02665 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Orangutan (94%), Gibbon (94%), Elephant (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EMR3 Antibody

• egf-like module containing, mucin-like, hormone receptor-like 3
• EGF-like module receptor 3
• EGF-like module-containing mucin-like hormone receptor-like 3
• egf-like module-containing mucin-like receptor 3
• EMR3

Background

EMR3 is an Orphan-B GPCR with an unknown ligand. Two variants are produced by alternative splicing. One form encodes a 652-amino acid GPCR consisting of two EGF-like domains and a mucin stalk, while the other is a truncated soluble form containing only two EGF-like domains. EMR3 and EMR2 share a high degree of sequence identity in the transmembrane domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADGRE3
• Entrez:
  • 84658(Human)
• Uniprot:
  • Q9BY15()