EMILIN2 Antibody (1031401) [Unconjugated]

EMILIN2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Mouse Anti-Human EMILIN2 Monoclonal Antibody (Catalog # MAB10590) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1031401
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EMILIN2 Antibody (1031401) [Unconjugated] Summary

• Immunogen: Human EMILIN2 synthetic peptide
• Specificity: Detects human EMILIN2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMILIN2 Antibody (1031401) [Unconjugated]

• Elastin microfibril interface-located protein 2
• elastin microfibril interfacer 2EMILIN-2
• EMIL2
• EMILIN2
• extracellular glycoprotein EMILIN-2
• FLJ33200
• FOAP-10
• Protein FOAP-10

Background

EMILIN (Elastin Microfibril Interface Located Protein) is an elastic fiber-associated glycoprotein.  EMILIN2 is a protein that may be responsible for anchoring smooth muscles to elastin fibers.  It also may be involved in the process that regulates vessel assembly. MRNA expression of EMILIN2 is more restricted compared to EMILIN1 with highest levels being present in fetal heart and adult lung.  EMILIN2 is secreted extracellularly by in vitro-grown cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.