EGR2 Overexpression Lysate

Images

 
Western Blot: EGR2 Overexpression Lysate (Adult Normal) [NBP2-09030] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EGR2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EGR2 Overexpression Lysate Summary

Description
Transient overexpression lysate of early growth response 2 (EGR2), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC212183 and based on accession number NM_000399. The protein contains a C-MYC/DDK Tag.
Gene
EGR2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EGR2 Overexpression Lysate

  • AT591
  • CMT1D
  • CMT4E
  • early growth response 2 (Krox-20 homolog, Drosophila)
  • early growth response 2
  • early growth response protein 2
  • EGR-2
  • FLJ14547
  • Krox-20 homolog, Drosophila
  • KROX-20, Drosophila, homolog (early growth response-2)
  • KROX20DKFZp686J1957
  • Zinc finger protein Krox-20

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2818
Species: Hu
Applications: WB, ICC
NB100-1607
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP2-82977
Species: Hu
Applications: WB, IHC
NB110-59086
Species: Hu, Pm, Bv(-), Mu(-), Rt(-)
Applications: WB, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, KO
NBP2-53381
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBL1-15012
Species: Hu
MAB6979
Species: Hu
Applications: WB
NBP2-20486
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-27559
Species: Hu
Applications: WB, PEP-ELISA
AF2864
Species: Hu
Applications: WB, ICC
NB600-717
Species: Hu, Mu, Rt, Po, Bv, Ch, GP, Ma, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NBP1-06032
Species: Hu
Applications: WB, PEP-ELISA
NBP2-19462
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
MAB3810
Species: Hu, Rt
Applications: WB, Simple Western, ICC

Publications for EGR2 Lysate (NBP2-09030) (0)

There are no publications for EGR2 Lysate (NBP2-09030).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EGR2 Lysate (NBP2-09030) (0)

There are no reviews for EGR2 Lysate (NBP2-09030). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EGR2 Lysate (NBP2-09030) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBP2-09030

Additional EGR2 Products

Bioinformatics Tool for EGR2 Lysate (NBP2-09030)

Discover related pathways, diseases and genes to EGR2 Lysate (NBP2-09030). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EGR2 Lysate (NBP2-09030)

Discover more about diseases related to EGR2 Lysate (NBP2-09030).
 

Pathways for EGR2 Lysate (NBP2-09030)

View related products by pathway.

PTMs for EGR2 Lysate (NBP2-09030)

Learn more about PTMs related to EGR2 Lysate (NBP2-09030).
 

Research Areas for EGR2 Lysate (NBP2-09030)

Find related products by research area.

Blogs on EGR2

There are no specific blogs for EGR2, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EGR2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol EGR2
COVID-19 update