EGR2 Antibody

Images

 
Western Blot: EGR2 Antibody [NB100-68146] - Analysis of EGR2 antibody using this antibody at 0.0 3ug/ml in HepG2 cell lysate.
Immunohistochemistry-Paraffin: EGR2 Antibody [NB100-68146] - (3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

EGR2 Antibody Summary

Immunogen
Peptide with sequence HGTAGPDRKPFPC corresponding to internal region according to NP_000390.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EGR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-6 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
Western blot: Approx 55kDa band observed in lysates of cell line HepG2 (calculated MW of 50.3kDa according to NP_000390.2).
IHC: In paraffin embedded Human Colon shows nuclear staining of absorptive cells. Data obtained by a previous batch (different goat).
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-68146.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human,Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EGR2 Antibody

  • AT591
  • CMT1D
  • CMT4E
  • early growth response 2 (Krox-20 homolog, Drosophila)
  • early growth response 2
  • early growth response protein 2
  • EGR-2
  • FLJ14547
  • Krox-20 homolog, Drosophila
  • KROX-20, Drosophila, homolog (early growth response-2)
  • KROX20DKFZp686J1957
  • Zinc finger protein Krox-20

Background

ERG2 is a sequence-specific transcription factor, that binds to two specific DNA sites located in the promoter region of HOXA4. Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN), which can be inherited in either an autosomal dominant or recessive manner. Recessive CHN can present as Charcot-Marie-Tooth disease type 4E, type 1D, or Dejerine-Sottas syndrome (DSS), a group of neuropathies associated with decreased nerve conduction velocities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGR2 Antibody (NB100-68146)(1)

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Bioinformatics

Gene Symbol EGR2
Entrez
Uniprot