DOK5 Antibody

Western Blot: DOK5 Antibody [NB100-1463] - Analysis of DOK5 in Human Kidney lysate (35 ug protein in RIPA buffer) using this antibody at 0.1 ug/ml.

Immunohistochemistry-Paraffin: DOK5 Antibody [NB100-1463] - IHC staining of paraffin embedded Human Tonsil using this antibody at 1 ug/ml. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

Western Blot: DOK5 Antibody [NB100-1463] - HEK293 overexpressing DOK5 and probed with (mock transfection in first lane).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DOK5 Antibody Summary

• Immunogen: Peptide with sequence CLHRTETFPAYRSEH corresponding to C-Terminus according to NP_060901.2.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DOK5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing DOK5 a band of approx. 38 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5 kDa band according to NP_060901.2. IHC-P: Tonsil stains scattered cells in the interfollicular area compatible with mast cells.
• Control:

  DOK5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DOK5 Antibody

• C20orf180
• chromosome 20 open reading frame 180
• dJ805C22.1
• docking protein 5
• Downstream of tyrosine kinase 5
• Insulin receptor substrate 6
• IRS6
• IRS-6
• MGC16926

Background

DOK5 is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP. Two transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DOK5 Antibody (NB100-1463)(1)

Publication using NB100-1463

• Cai D, Dhe-Paganon S, Melendez PA et al. Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5. J Biol Chem 2003-07-11 [PMID: 12730241]

Bioinformatics

• Gene Symbol: DOK5
• Entrez:
  • 55816(Human)
  • 76829(Mouse)
• Uniprot:
  • NP_060901(Human)