DNAJC8 Antibody (SR2404)

Western Blot: DNAJC8 Antibody (SR2404) [NBP3-27696] - Western blot analysis of mouse brain, rat lung lysates

Western Blot: DNAJC8 Antibody (SR2404) [NBP3-27696] - Western blot analysis of Jurkat, Hela, COS7, Raji lysates

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC, IP
• Clone: SR2404
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

DNAJC8 Antibody (SR2404) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human DNAJC8 (Uniprot #: O75937)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DNAJC8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunoprecipitation 1:20
  • Western Blot 1:500-1:1000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for DNAJC8 Antibody (SR2404)

• DnaJ (Hsp40) homolog, subfamily C, member 8
• dnaJ homolog subfamily C member 8
• HSPC331
• SPF31Splicing protein spf31

Background

DjC8 (DnaJ subfamily C member 8) belongs to the Hsp40 (heat shock protein 40) family of proteins that function as co-chaperones to Hsp70.Hsp40 proteins are classified into 3 main subfamilies (A, B, and C). The families are defined by the presence of particular domains which include a highly conserved alpha-helical N-terminal domain termed the J-domain, a glycine/phenylalanine-rich region, a cysteine-rich region, and a C-terminal beta-sheet containing domain. Djc8 is part of subfamily C that bears only the J-domain. DjC8 is also known as splicing protein spf31, and DNAJC8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DNAJC8