![Western Blot: DIAPH3 Antibody [NBP2-80419] - Analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize2/DIAPH3 Antibody-Immunohistochemistry-Paraffin-NBP2-80419-img0007.jpg "Western Blot: DIAPH3 Antibody [NBP2-80419] - Analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide within human DIAPH3 aa 1-50. |
| Localization | Cytosol. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DIAPH3 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 137 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1X TBS(pH7.4), 0.5%BSA, 50%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
Diseases for DIAPH3 Antibody (NBP2-80419)Discover more about diseases related to DIAPH3 Antibody (NBP2-80419).
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PTMs for DIAPH3 Antibody (NBP2-80419)Learn more about PTMs related to DIAPH3 Antibody (NBP2-80419).
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| Gene Symbol | DIAPH3 |
![Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/DIAPH3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80419-img0004.jpg "Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human kidney tissue using DIAPH3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/DIAPH3-Antibody-Immunohistochemistry-Paraffin-NBP2-80419-img0006.jpg "Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human kidney tissue using DIAPH3 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: DIAPH3 Antibody [NBP2-80419] - Analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/DIAPH3-Antibody-Flow-Cytometry-NBP2-80419-img0001.jpg "Flow Cytometry: DIAPH3 Antibody [NBP2-80419] - Analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/DIAPH3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80419-img0002.jpg "Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/DIAPH3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80419-img0003.jpg "Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human placenta tissue using DIAPH3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/DIAPH3-Antibody-Immunohistochemistry-Paraffin-NBP2-80419-img0005.jpg "Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human placenta tissue using DIAPH3 antibody. Counter stained with hematoxylin.")
![Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/images2/DIAPH1-Antibody-Western-Blot-NB600-231-img0009.jpg)
![Immunohistochemistry: DIAPH1 Antibody [NB600-231] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Diaphanous used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/images2/DIAPH1-Antibody-Immunohistochemistry-NB600-231-img0010.jpg)
![Western Blot: DIAPH1 Antibody [NB600-231] - Whole cell lysate (50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/images2/DIAPH1-Antibody-Western-Blot-NB600-231-img0008.jpg)
![Western Blot: DIAPH-2 Antibody [NBP1-85217] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/DIAPH-2-Antibody-Western-Blot-NBP1-85217-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: DIAPH-2 Antibody [NBP1-85217] - Staining of human cell line U-251 MG shows localization to nucleoli, endoplasmic reticulum and vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/DIAPH-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85217-img0016.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0015.jpg)
![Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/images2/RhoD-Antibody-Western-Blot-NBP1-58359-img0002.jpg)
![Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos](http://images.novusbio.com/images2/RhoD-Antibody-Immunohistochemistry-NBP1-58359-img0004.jpg)
![Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.](http://images.novusbio.com/images2/RhoD-Antibody-Immunohistochemistry-Paraffin-NBP1-58359-img0003.jpg)
![Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/images2/Rhodopsin Antibody (1D4)-Western Blot-NBP1-47602-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.](http://images.novusbio.com/images2/Rhodopsin-Antibody-(1D4)-Immunocytochemistry-Immunofluorescence-NBP1-47602-img0003.jpg)
![Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.](http://images.novusbio.com/images2/RhoA-Antibody-Western-Blot-NB100-91273-img0034.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/images2/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0024.jpg)
![Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.](http://images.novusbio.com/images2/RhoA Antibody-Immunohistochemistry-NB100-91273-img0040.jpg)
![ELISA: FMN1 Antibody [44110002]](http://images.novusbio.com/images/44110002-T01565A01.jpg)
![Western Blot: ACTR2 Antibody (1B10-C4) [H00010097-M01] - ACTR2 monoclonal antibody (M01), clone 1B10-C4 Analysis of ACTR2 expression in Hela S3 NE.](http://images.novusbio.com/images2/ACTR2-Antibody-(1B10-C4)-Western-Blot-H00010097-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Analysis of monoclonal antibody to ACTR2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/ACTR2-Antibody-(1B10-C4)-Immunohistochemistry-Paraffin-H00010097-M01-img0007.jpg)
![Western Blot: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Western Blot analysis of ACTR2 expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/images2/ACTR2-Antibody-(1B10-C4)-Western-Blot-H00010097-M01-img0002.jpg)
![Western Blot: FMNL3 Antibody [NBP2-24724] - Analysis of Formin-like protein 3 in human brain lysate using this antibody. 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/images2/FMNL3-Antibody-Western-Blot-NBP2-24724-img0003.jpg)
![Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/images2/AICDA Antibody-Western Blot-NBP1-30955-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/AICDA Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30955-img0002.jpg)
![Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/images2/AICDA-Antibody-Immunohistochemistry-Paraffin-NBP1-30955-img0005.jpg)
![Western Blot: FMNL1 Antibody [NBP1-33431] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/FMNL1 Antibody-Western Blot-NBP1-33431-img0003.jpg)
![Immunohistochemistry-Paraffin: FMNL1 Antibody [NBP1-33431] - Paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/FMNL1 Antibody-Immunohistochemistry-Paraffin-NBP1-33431-img0002.jpg)
![Western Blot: FMNL1 Antibody [NBP1-33431] - H1299 lysates (Lane A) and Hela cells (Lane B) using NBP1-33431 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel.](http://images.novusbio.com/images2/FMNL1 Antibody-Western Blot-NBP1-33431-img0001.jpg)
![Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of CDC42 on different lysates using anti-CDC42 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: HepG2](http://images.novusbio.com/images2/CDC42 Antibody (JJ086-04)-Western Blot-NBP2-67895-img0005.jpg)
![Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/CDC42-Antibody-(JJ086-04)-Immunohistochemistry-Paraffin-NBP2-67895-img0004.jpg)
![Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/CDC42-Antibody-(JJ086-04)-Flow-Cytometry-NBP2-67895-img0001.jpg)
![Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.](http://images.novusbio.com/images/VASP Antibody (4D6)-Western Blot-NBP2-00555-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP.](http://images.novusbio.com/images/VASP Antibody (4D6)-Immunocytochemistry-Immunofluorescence-NBP2-00555-img0010.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymphoma tissue using anti-VASP mouse monoclonal antibody.](http://images.novusbio.com/images/VASP Antibody (4D6)-Immunohistochemistry-Paraffin-NBP2-00555-img0009.jpg)
![Western Blot: Filamin B Antibody [NBP1-90286] - Analysis in human cell lines A-549 and HEK293 using anti-FLNB antibody. Corresponding FLNB RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/images2/Filamin-B-Antibody-Western-Blot-NBP1-90286-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Filamin B Antibody [NBP1-90286] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/Filamin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90286-img0015.jpg)
![Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human skin shows weak to moderate cytoplasmic positivity in keratinocytes.](http://images.novusbio.com/images2/Filamin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90286-img0026.jpg)
![Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] Analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.](http://images.novusbio.com/images/CYB5R3 Antibody (2A10)-Western Blot-NBP2-02705-img0013.jpg)
![Immunohistochemistry-Paraffin: CYB5R3 Antibody (2A10) [NBP2-02705] - Staining of paraffin-embedded Human liver tissue using anti-CYB5R3 mouse monoclonal antibody.](http://images.novusbio.com/images/CYB5R3 Antibody (2A10)-Immunohistochemistry-Paraffin-NBP2-02705-img0012.jpg)
![Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYB5R3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images/CYB5R3 Antibody (2A10)-Flow Cytometry-NBP2-02705-img0002.jpg)
![Western Blot: Serum Response Factor SRF Antibody [NBP1-33063] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/images2/Serum-Response-Factor-SRF-Antibody-Western-Blot-NBP1-33063-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody [NBP1-33063] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Serum-Response-Factor-SRF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33063-img0003.jpg)
![Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-33063] - Mouse lung SRF dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/images2/Serum-Response-Factor-SRF-Antibody-Immunohistochemistry-Paraffin-NBP1-33063-img0008.jpg)
![Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against human RHOF (AA: 1-84) recombinant protein. (Expected MW is 34.9 kDa)](http://images.novusbio.com/images2/RhoF-Antibody-(1D4B6)-Western-Blot-NBP2-52553-img0002.jpg)
![Flow Cytometry: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis of Hela cells using RHOF mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/RhoF-Antibody-(1D4B6)-Flow-Cytometry-NBP2-52553-img0001.jpg)
![Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against HEK293 (1) and RHOF (AA: 1-84)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/RhoF Antibody (1D4B6)-Western Blot-NBP2-52553-img0003.jpg)
![Western Blot: DAAM1 Antibody [NBP2-24623] - Analysis of DAAM1 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24623 5.0 ug/ml.](http://images.novusbio.com/images2/DAAM1-Antibody-Western-Blot-NBP2-24623-img0002.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")