DIAPH3 Antibody

Images

 
Western Blot: DIAPH3 Antibody [NBP2-80419] - Analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human kidney tissue using DIAPH3 antibody. Counter stained with hematoxylin.
Flow Cytometry: DIAPH3 Antibody [NBP2-80419] - Analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor ...read more
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human placenta tissue using DIAPH3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Product Discontinued
View other related DIAPH3 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-80419
    • Availability
      Product Discontinued

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DIAPH3 Antibody Summary

Immunogen
Synthetic peptide within Human DIAPH3 aa 1,059-1,108 / 1,193. (SwissProt: Q9NSV4 Human)
Localization
Cytosol.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIAPH3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
Theoretical MW
137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH7.4), 0.2% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DIAPH3 Antibody

  • DIAP3
  • diaphanous homolog 3 (Drosophila)
  • diaphanous-related formin 3
  • DKFZp434C0931
  • DKFZp686A13178
  • homolog) 3
  • mDia2
  • protein diaphanous homolog 3

Background

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to themembrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, andtranscriptional activation of the serum r

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional DIAPH3 Products

Bioinformatics Tool for DIAPH3 Antibody (NBP2-80419)

Discover related pathways, diseases and genes to DIAPH3 Antibody (NBP2-80419). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for DIAPH3 Antibody (NBP2-80419)

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Research Areas for DIAPH3 Antibody (NBP2-80419)

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Blogs on DIAPH3

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Contact Information

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Product PDFs

Calculators

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Bioinformatics

Gene Symbol DIAPH3