DIAPH3 Antibody

Images

 
Western Blot: DIAPH3 Antibody [NBP2-80419] - Analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human kidney tissue using DIAPH3 antibody. Counter stained with hematoxylin.
Flow Cytometry: DIAPH3 Antibody [NBP2-80419] - Analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor ...read more
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human placenta tissue using DIAPH3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DIAPH3 Antibody Summary

Immunogen
Synthetic peptide within human DIAPH3 aa 1-50.
Localization
Cytosol.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIAPH3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1X TBS(pH7.4), 0.5%BSA, 50%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for DIAPH3 Antibody

  • DIAP3
  • diaphanous homolog 3 (Drosophila)
  • diaphanous-related formin 3
  • DKFZp434C0931
  • DKFZp686A13178
  • homolog) 3
  • mDia2
  • protein diaphanous homolog 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol DIAPH3