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DIAPH3 Antibody

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Western Blot: DIAPH3 Antibody [NBP2-80419] - Analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
Western Blot: DIAPH3 Antibody [NBP2-80419] - Analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human kidney tissue using DIAPH3 antibody. Counter stained with hematoxylin.
Flow Cytometry: DIAPH3 Antibody [NBP2-80419] - Analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor ...read more
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunocytochemistry/ Immunofluorescence: DIAPH3 Antibody [NBP2-80419] - Staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% ...read more
Immunohistochemistry-Paraffin: DIAPH3 Antibody [NBP2-80419] - Analysis in human placenta tissue using DIAPH3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-80419
    • Availability
      Product Discontinued

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DIAPH3 Antibody Summary

Immunogen
Synthetic peptide within Human DIAPH3 aa 1,059-1,108 / 1,193. (SwissProt: Q9NSV4 Human)
Localization
Cytosol.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIAPH3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
Theoretical MW
137 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH7.4), 0.2% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DIAPH3 Antibody

  • DIAP3
  • diaphanous homolog 3 (Drosophila)
  • diaphanous-related formin 3
  • DKFZp434C0931
  • DKFZp686A13178
  • homolog) 3
  • mDia2
  • protein diaphanous homolog 3

Background

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to themembrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, andtranscriptional activation of the serum r

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: DIAPH1 Antibody [NB600-231] - Whole cell lysate (50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 ug/ml.
Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human placenta shows high expression.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.
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Immunohistochemistry-Paraffin: RhoD Antibody [NBP1-58359] - Human Tonsil Tissue, 4-8ug/ml.
Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry: RhoD Antibody [NBP1-58359] - Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X.
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Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug.. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000.
Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..
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Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.
Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
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Applications: ELISA, ICC/IF, WB
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Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of Jurkat cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Flow Cytometry: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYB5R3 antibody, and then analyzed by flow cytometry.
Western Blot: CYB5R3 Antibody (2A10) [NBP2-02705] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3.
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Serum Response
Factor SRF Antibody ( ...
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Flow Cytometry: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Flow cytometric analysis of PFA-fixed HeLa cells. Serum Response Factor SRF antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Serum Response Factor SRF Antibody (PCRP-SRF-1F7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody (PCRP-SRF-1F7) [NBP3-13785] - IHC analysis of formalin-fixed, paraffin-embedded human colon. Strong nuclear staining using Serum Response Factor SRF antibody (PCRP-SRF-1F7) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
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RhoF Antibody
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ELISA: RhoF Antibody (1D4B6) [NBP2-52553] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Flow Cytometry: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis of Hela cells using RHOF mouse mAb (green) and negative control (red).
Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against HEK293 (1) and RHOF (AA: 1-84)-hIgGFc transfected HEK293 (2) cell lysate.
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Proximity Ligation Assay: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of protein-protein interactions between RHOA and DAAM1. HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in PC-12 (Cat # L012V1).
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Bioinformatics

Gene Symbol DIAPH3