DGK-zeta Antibody (RB3716)

Western Blot: DGK-zeta Antibody [NBP2-29920] - DGKZ (arrow) using DGKZ Antibody (C-term) (NBP2-29920). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DGKZ

Immunocytochemistry/Immunofluorescence: DGK-zeta Antibody [NBP2-29920] - SF1 colocalizes with DGK and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and anti-DGKZ (B) for 1 h. Coverslips

Immunohistochemistry-Paraffin: DGK-zeta Antibody [NBP2-29920] - Human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Western Blot: DGK-zeta Antibody [NBP2-29920] - Ramos cell line lysates (35ug/lane).This demonstrates the DGKZ antibody detected the DGKZ protein (arrow).

Product Discontinued

DGK-zeta Antibody (RB3716) Summary

• Immunogen: This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 989-1019 amino acids from the C-terminal region of human DGKZ.
• Localization: Cellular Location Cytoplasm. Nucleus. Cell membrane. Tissue Location Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DGKZ
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Western Blot 1:1000
• Theoretical MW: 124.128 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for DGK-zeta Antibody (RB3716)

• DAGK5
• DAGK6DAG kinase zeta
• DGKZ
• DGKzeta
• DGK-zeta
• diacylglycerol kinase zeta
• diacylglycerol kinase, zeta 104kDa
• diacylglycerol kinase, zeta
• Diglyceride kinase zeta
• EC 2.7.1.107
• hDGKzeta

Background

DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKZ
• Uniprot:
  • Q13574(Human)