DGK-beta Antibody (646523)

Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DGK-beta Monoclonal Antibody (Catalog # MAB6727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-beta at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

• Reactivity: Hu
• Applications: WB
• Clone: 646523
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DGK-beta Antibody (646523) Summary

• Immunogen: E. coli-derived recombinant human DGK-beta
  Met1-Ser143
  Accession # Q9Y6T7
• Specificity: Detects human DGK-beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DGK-alpha , gamma , epsilon , iota , kappa , theta , or zeta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DGKB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-beta Antibody (646523)

• 90 kDa diacylglycerol kinase
• DAG kinase beta
• DAGK2
• DGKB
• DGKbeta
• DGK-beta
• diacylglycerol kinase beta
• diacylglycerol kinase, beta (90kD)
• diacylglycerol kinase, beta 90kDa
• Diglyceride kinase beta
• EC 2.7.1
• EC 2.7.1.107
• KIAA0718DGKDGK-BETA

Background

Diacylglycerol kinase beta (DGK-beta ) is an approximately 90 kDa kinase that phosphorylates the lipid diacylglycerol. It is expressed in the brain and colocalizes with D1 and D2 dopamine receptors. DGK-beta promotes the formation and maturation of dendritic spines. It contains two EF-hand motifs (aa 149-184 and 194-229), two zinc finger regions (aa 244-294 and aa 309-358), and a catalytic domain (aa 434-568). Alternate splicing generates an additional isoform with a 31 aa C-terminal deletion. Over aa 1-143, human DGK-beta shares 97% aa sequence identity with mouse and rat DGK-beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKB
• Uniprot:
  • Q9Y6T7()