Deoxyribonuclease I like 1 Antibody (4E8) - Azide and BSA Free

Western Blot: Deoxyribonuclease I like 1 Antibody (4E8) [H00001774-M02] - Analysis of DNASE1L1 expression in transfected 293T cell line by DNASE1L1 monoclonal antibody (M02), clone 4E8.Lane 1: DNASE1L1 transfected lysate(33.9 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Deoxyribonuclease I like 1 Antibody (4E8) [H00001774-M02] - Detection limit for recombinant GST tagged DNASE1L1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Deoxyribonuclease I like 1 Antibody (4E8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DNASE1L1 (AAH01561, 18 a.a. ~ 302 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFRICAFNAQRLTLAKVAREQVMDTLVRILARCDIMVLQEVVDSSGSAIPLLLRELNRFDGSGPYSTLSSPQLGRSTYMETYVYFYRSHKTQVLSSYVYNDEDDVFAREPFVAQFSLPSNVLPSLVLVPLHTTPKAVEKELNALYDVFLEVSQHWQSKDVILLGDFNADCASLTKKRLDKLELRTEPGFHWVIADGEDTTVRASTHCTYDRVVLHGERCRSLLHTAAAFDFPTSFQLTEEEALNISDHYPVEVELKLSQAHSVQPLSLTVLLLLSLLSPQLCPAA
• Specificity: DNASE1L1 - deoxyribonuclease I-like 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DNASE1L1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Deoxyribonuclease I like 1 Antibody (4E8) - Azide and BSA Free

• deoxyribonuclease I-like 1muscle-specific
• DNase I-like 1
• DNase X
• DNASEX
• DNL1LEC 3.1.21.-
• EC 3.1.21
• EC 3.1.21.1
• Muscle-specific DNase I-like

Background

This gene encodes a member of the deoxyribonuclease family and the protein shows high sequence similarity to lysosomal DNase I. Alternate transcriptional splice variants, encoding the same protein, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Deoxyribonuclease I like 1 Antibody (H00001774-M02)(2)

Publications using H00001774-M02

• Kaushik P, Yuanchang Z, Yongliang W et al. Ubiquitous membrane-bound DNase activity in podosomes and invadopodia. J Cell Biol. 2021-04-05 [PMID: 33904858]
• Wang Y, Zhao Y, Sarkar A, Wang X et al. Optical sensor revealed abnormal nuclease spatial activity on cancer cell membrane. J Biophotonics. 2018-11-29 [PMID: 30488667]

Bioinformatics

• Gene Symbol: DNASE1L1
• Entrez:
  • 1774(Human)
• OMIM:
  • 300081(Human)
• Uniprot:
  • AAH01561(Human)