DEF8 Antibody (OTI4B6) - Azide and BSA Free

Western Blot: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF8.

Immunocytochemistry/Immunofluorescence: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF8.

Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human lymph node tissue using anti-DEF8 mouse monoclonal antibody.

Flow Cytometry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Analysis of Hela cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DEF8 mouse monoclonal antibody.

Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DEF8 mouse monoclonal antibody.

Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human liver tissue using anti-DEF8 mouse monoclonal antibody.

Immunohistochemistry: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Staining of paraffin-embedded Human lymphoma tissue using anti-DEF8 mouse monoclonal antibody.

CyTOF-ready: DEF8 Antibody (OTI4B6) - Azide and BSA Free [NBP2-71969] - Analysis of Jurkat cells, using anti-DEF8 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI4B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

DEF8 Antibody (OTI4B6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DEF8 Antibody (OTI4B6) - Azide and BSA Free (NBP2-00574) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human DEF8 (NP_997397) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DEF8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 58.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for DEF8 Antibody (OTI4B6) - Azide and BSA Free

• DEF-8
• differentially expressed in FDCP 8 homolog (mouse)
• differentially expressed in FDCP 8 homolog
• FLJ20186
• MGC104349

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DEF8