DCLK3 Antibody

Western Blot: DCLK3 Antibody [21890002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 74 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Immunohistochemistry: DCLK3 Antibody [21890002] - Normal tissues generally displayed weak to moderate cytoplasmic and occasional nuclear positivity. Ciliated cells in airways and fallopian tube exhibited distinct membranous staining. Cells for example in liver, prostate and spleen were negative. Colo-rectal and endometrial cancers exhibited some moderately stained cases while malignant gliomas, ovarian and stomach cancers showed several weakly immunoreactive samples. Remaining malignant tissues were mainly negative. Image and statement courtesy of the Human Protein Atlas (HPA).

Western Blot: DCLK3 Antibody [21890002] - Western Blot was performed using affinity purified SEQer DCAMKL3 antibody, aa(138-237) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer DCAMKL3 antibody, aa(138-237) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.

ELISA: DCLK3 Antibody [21890002]

Product Discontinued

DCLK3 Antibody Summary

• Immunogen: This antibody is specific for the N Terminus Region of the target protein.
• Specificity: This product is specific for Human DCAMKL3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCLK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for DCLK3 Antibody

• CLICK-I
• DCAMKL3
• DCDC3CCLR
• doublecortin and CaM kinase-like 3
• Doublecortin domain-containing protein 3C
• Doublecortin-like and CAM kinase-like 3
• doublecortin-like kinase 3EC 2.7.11.1
• EC 2.7.11
• II-related
• KIAA1765DCK3
• serine/threonine-protein kinase DCLK3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DCLK3 Antibody (21890002)(3)

Publications using 21890002

• Galvan L, Francelle L, Gaillard MC et al. The striatal kinase DCLK3 produces neuroprotection against mutant huntingtin. Brain. 2018-03-09 [PMID: 29534157] (IF/IHC, Human)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: DCLK3
• Uniprot:
  • XM_047355(Human)