Cysteine Conjugate beta-Lyase/CCBL1 Antibody

Product Discontinued

Cysteine Conjugate beta-Lyase/CCBL1 Antibody Summary

• Immunogen: Peptide with sequence C-DISDFKRKMPD corresponding to internal region according to NP_004050.3, NP_001116144.1.
• Epitope: DISDFKRKMPD
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004050.3; NP_001116144.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KYAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments in human brain (cerebral and frontal cortex) and human breast cancer lysates gave no specific signal but low background.
• Control:

  Cysteine Conjugate beta-Lyase/CCBL1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Cysteine Conjugate beta-Lyase/CCBL1 Antibody

• CCBL1
• Cysteine Conjugate betaLyase
• Cysteine Conjugate beta-Lyase
• cysteine conjugate-beta lyase, cytoplasmic
• cysteine conjugate-beta lyase; cytoplasmic (glutamine transaminase K
• Cysteine-S-conjugate beta-lyase
• EC 2.6.1.64
• EC 2.6.1.7
• EC 4.4.1.13
• FLJ95217
• Glutamine Transaminase K
• glutamine-phenylpyruvate aminotransferase
• Glutamine--phenylpyruvate transaminase
• GTKMGC29624
• KAT1
• KATI
• KATIbeta-lysase, kidney
• Kyneurenine Aminotransferase I
• kyneurenine aminotransferase
• kyneurenine aminotransferase)
• Kynurenine aminotransferase I
• kynurenine--oxoglutarate transaminase 1
• Kynurenine--oxoglutarate transaminase I

Background

CCBL1 is a cytosolic enzyme which is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism leads to the formation of reactive metabolites which can lead to nephrotoxicity and neurotoxicity. CCBL1 catalyzes the irreversible transamination of the L-tryptophan metabolite L-kinurenine to form kynurenic acid (KA). It catalyzes the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond. This enzyme has a central role in the bioactivation of nephrotoxic halogenated hydrocarbons the possibility of human exposure to these chemicals has focused interest on the CCBL1 from human kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cysteine Conjugate beta-Lyase/CCBL1 Antibody (NB100-79870)(1)

Publication using NB100-79870

• Cooper AJ. The role of glutamine transaminase K (GTK) in sulfur and alpha-keto acid metabolism in the brain, and in the possible bioactivation of neurotoxicants. Neurochem Int 2004-06-01 [PMID: 15016471]

Bioinformatics

• Gene Symbol: KYAT1
• Entrez:
  • 883(Human)
• Uniprot:
  • NP_004050.3(Human)