Recombinant Human Cyclophilin B Protein

SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human Cyclophilin B Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 26- 216 of Human Cyclophilin B
  
  

  Source: E.coli

  Amino Acid Sequence: MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE

• Details of Functionality: Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PPIB
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 21.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer (pH 8.0), 20mM NaCl, 0.5mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Cyclophilin B Protein

• Cyclophilin B
• cyclophilin-like protein
• CYPB
• CYPBEC 5.2.1.8
• CYP-S1
• MGC2224
• OI9MGC14109
• peptidyl-prolyl cis-trans isomerase B
• peptidylprolyl isomerase B (cyclophilin B)
• PPIase B
• PPIB
• Rotamase B
• S-cyclophilin
• SCYLP

Background

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Cyclophilin B Recombinant Protein (NBC1-18424)(3)

Publications using NBC1-18424

• Wang G, Li Y, Wang T et al. Multi-comparative Thermal Proteome Profiling Uncovers New O-GlcNAc Proteins in a System-wide Method Analytical chemistry 2022-12-29 [PMID: 36580660]
• Eduard Sarró, Mónica Durán, Ana Rico, Diana Bou-Teen, Vanesa Fernández-Majada, Anthony J Croatt, Karl A Nath, Maria Teresa Salcedo, Justin H Gundelach, Daniel Batlle, Richard J Bram, Anna Meseguer, Xuebiao Yao Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Journal of Molecular Cell Biology 2020-07-01 [PMID: 32162654]
• Zhu D, Wang Z, Zhao JJ et al. The Cyclophilin A-CD147 complex promotes the proliferation and homing of multiple myeloma cells. Nat. Med. 2015-05-25 [PMID: 26005854] (Human)

Bioinformatics

• Gene Symbol: PPIB