Cyclophilin A Antibody (817815) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clone: 817815
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 555

Cyclophilin A Antibody (817815) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human Cyclophilin A in direct ELISAs and Western blots. Detects mouse and rat Cyclophilin in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Cyclophilin E, less than 15% cross-reactivity with rhCyclophilin F, and less than 5% cross-reactivity with rhCyclophilin A-like 4 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclophilin A Antibody (817815) [Alexa Fluor™ Plus 555]

• Cyclophilin A
• Cyclosporin A-binding protein
• CYPA
• CYPAMGC12404
• CYPH
• EC 5.2.1.8
• MGC117158
• MGC23397
• peptidyl-prolyl cis-trans isomerase A
• peptidylprolyl isomerase A (cyclophilin A)
• PPIA
• PPIase A
• Rotamase A
• Rotamase
• T cell cyclophilin

Background

Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins (1). Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity (2). The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity (3) and prevent incorporation of cyclophilin into viral particles (4). The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin (3, 5), making it a useful inhibitor for studying calcineurin activity.

1. Hamilton, G.S. and J.P. Steiner  (1998) J. Med. Chem. 41:5119.
2. Cantin, R. et al. (2005) J. Virology 79:6577.
3. Liu, J. et al. (1992) Biochemistry 31:3896.
4. Wiegers K. and H.G. Krausslich (2002) Virology 294:289.
5. Liu, J. et al. (1991) Cell 66:807.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.