CRYBA4 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

CRYBA4 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CRYBA4.

  Source: E. coli

  Amino Acid Sequence: MTLQCTKSAGPWKMVVWDEDGFQG

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: CRYBA4
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-57040.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for CRYBA4 Recombinant Protein Antigen

• Beta-A4 crystallin
• beta-crystallin A4
• crystallin, beta A4
• crystallin, beta polypeptide A4
• eye lens structural protein
• MCOPCT4

Background

CRYBA4, or Beta-crystallin A4, consists of a 196 amino acid isoform that is 22 kDa, and is involved in maintaining the structure, transparency, and refractive index of the eye lens. Current disease research has linked defects in CRYBA4 to several types of cataracts, including lamellar 2 cataracts, zonular cataract, congenital cataracts, and microphthalmia cataract. Researchers are also studying the relation between the protein and the disorders choroididits and neurofibromatosis. CRYBA4 is included in several biological processes, including visual perception and camera-type eye development, through which it interacts with CRYBB2 and CRYBB3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CRYBA4