Connexin 32/GJB1 Antibody (GJB1/1753) [Allophycocyanin]

Product Discontinued

Connexin 32/GJB1 Antibody (GJB1/1753) [Allophycocyanin] Summary

• Immunogen: Recombinant human Connexin 32/GJB1 protein (Uniprot: P08034)
• Localization: Cytoplasmic and Cell Surface
• Specificity: This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: GJB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Connexin 32/GJB1 Antibody (GJB1/1753) [Allophycocyanin]

• CMTX
• CMTX1
• Connexin 32
• Connexin-32
• CX32
• CX32beta 1, 32kD (connexin 32, Charcot-Marie-Toothneuropathy, X-linked)
• gap junction beta-1 protein
• gap junction protein, beta 1, 32kDa (connexin 32)
• gap junction protein, beta 1, 32kDa (connexin 32, Charcot-Marie-Toothneuropathy, X-linked)
• gap junction protein, beta 1, 32kDa
• GJB1

Background

Gap junctions are aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control and developmental regulation. The gap junction channels allow intercellular exchange of ions, nucleotides and small molecules between adjacent cells. Unlike other membrane channels, intercellular channels span two plasma membranes and require the contribution of hemichannels, called connexons, from both participating cells. These channels are permeate to molecules as large as 1 kD, and they have been detected in virtually every cell type in mammals, except mature skeletal muscle, spermatozoa and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins, which are termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins ranging between molecular weights of 26 and 70 kD. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group to which Cx26, Cx30, Cx31, Cx31.1 and Cx32 belong, and the other termed class II or alpha group, represented by Cx33, Cx37, Cx40, Cx43 and Cx46.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GJB1