Human Connexin 26/GJB2 ELISA Kit (Colorimetric)

ELISA: Human Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79811] - Samples were spiked with high concentrations of Human Connexin 26/GJB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79811] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, and other biological fluids.
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Connexin 26/GJB2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CX26 in samples. No Significant cross-reactivity or interference between Human CX26 and analogues was observed.
• Standard Curve Range: 0.31-20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.86%
• Intra-Assay: CV% < 5.91%
• Spike Recovery: 91-108%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GJB2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Connexin 26/GJB2 ELISA Kit (Colorimetric)

• beta 2, 26kD (connexin 26)
• Connexin 26
• CX26
• CX26DFNA3
• DFNA3
• DFNA3A
• DFNB1
• gap junction protein, beta 2, 26kDa (connexin 26)
• gap junction protein, beta 2, 26kDa
• GJB2
• HID
• KID
• NSRD1
• PPK

Background

Connexin-26 / GJB2 is a gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-26 belongs to the class 1 (beta) group of connexins. Mutations in Connexin-26 are associated with genetically derived hearing impairments, including autosomal recessive non syndromic deafness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Connexin 26/GJB2 ELISA Kit (NBP2-79811)(1)

Publication using NBP2-79811

• Dwivedi AK, Gornalusse GG, Siegel DA et al. A cohort-based study of host gene expression: tumor suppressor and innate immune/inflammatory pathways associated with the HIV reservoir size PLoS pathogens 2023-11-29 [PMID: 38019897] (ELISA)

FAQs for Connexin 26/GJB2 ELISA Kit (NBP2-79811). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GJB2