Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric)

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ELISA: Human hCG ELISA Kit (Colorimetric) [NBP2-60038]
Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) [NBP2-60038] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, urine and cell culture supernates
Standard Curve Range
8 mIU/mL - 240 mIU/mL
Sensitivity
2 mIU/mL

Order Details

Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of human HCG. No significant cross-reactivity or interference between human HCG and analogues was observed.
Standard Curve Range
8 mIU/mL - 240 mIU/mL
Sensitivity
2 mIU/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CGB3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. HRP-conjugate
  4. Instruction manual
  5. Sample Diluent (for urine only, 10 x concentrate)
  6. Standard
  7. Stop Solution
  8. Substrate A
  9. Substrate B
  10. Wash Buffer (20 x concentrate)

Alternate Names for Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric)

  • CGB
  • CGB3
  • CGB3choriogonadotropin subunit beta
  • CGB5
  • CGB7
  • CGB8
  • CG-Beta
  • Choriogonadotropin Subunit Beta
  • Chorionic gonadotrophin chain beta
  • chorionic gonadotropin beta 3 subunit
  • Chorionic Gonadotropin beta Chain
  • chorionic gonadotropin beta subunit
  • chorionic gonadotropin, beta polypeptide
  • hCGB

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for HCG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CGB3