Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 1-5 hours |
Specificity |
This assay has high sensitivity and excellent specificity for detection of human HCG. No significant cross-reactivity or interference between human HCG and analogues was observed. |
Standard Curve Range |
8 mIU/mL - 240 mIU/mL |
Sensitivity |
2 mIU/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 15 |
Intra-Assay |
%CV < 15 |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
CGB3 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Strip (For 96 wells)
- Assay plate
- HRP-conjugate
- Instruction manual
- Sample Diluent (for urine only, 10 x concentrate)
- Standard
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (20 x concentrate)
|
Alternate Names for Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric)
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for HCG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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