CEP350 Antibody

Images

 
Independent Antibodies: Knockdown Validated: CEP350 Antibody [NB100-59811] - Representative WB of MDCKII cells with CAP350m and CAP350r (NB100-911) antibodies showing efficient depletion of CAP350 in cells ...read more
Independent Antibodies: Immunocytochemistry/ Immunofluorescence: CEP350 Antibody [NB100-59811] - Control MDCKII cells processed by IF with either anti-CAP350 rabbit antibody (CAP350r, top panels, NB100-911) or ...read more
Immunoprecipitation: CEP350 Antibody [NB100-59811] - Detection of Human CAP350 on HeLa whole cell lysate using NB100-59811. CAP350 was also immunoprecipitated by rabbit anti-CAP350 antibody NB100-59810.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ICC/IF, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CEP350 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 3066 and 3116 of human centrosome-associated protein 350 using the numbering given in entry NP_055625.3 (GeneID 9857).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CEP350
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:100-1:2000
Application Notes
This antibody is not recommended for crude preparations but can be used to blot CAP350 in enriched sources. ICC/IF, WB reactivity reported in (PMID: 20861314).
Theoretical MW
351 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-59811 in the following applications:

  • 5 publications
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 25764135). Mouse reactivity reported in scientific literature (PMID: 28625565).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CEP350 Antibody

  • CAP350GM133
  • centrosomal protein 350kDa
  • centrosome-associated protein 350
  • Centrosome-associated protein of 350 kDa
  • Cep350
  • FLJ38282
  • FLJ44058
  • KIAA0480centrosome associated protein 350

Background

CAP350 (Centrosome-Associated Protein 350) has been shown to localize to the centrosome as well as to dots in the pericentrosomal area. CAP350 functions to stabilize and anchor microtubules to the centrosome and participates in the maintenance of a pericentrosomal Golgi ribbon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEP350 Antibody (NB100-59811)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(5)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Canine
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CEP350
Entrez
Uniprot