CEP350 Antibody

Knockdown Validated: CEP350 Antibody [NB100-59811] - Representative WB of MDCKII cells with CAP350m and CAP350r (NB100-911) antibodies showing efficient depletion of CAP350 in cells infected with shCAP350 (shCAP) lentiviruses compared to those infected with control shm4 lentivirus. GMAP210 was used as a loading control. Alpha-catenin levels were also examined. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.1002087) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: CEP350 Antibody [NB100-59811] - Control MDCKII cells processed by IF with either anti-CAP350 rabbit antibody (CAP350r, top panels, NB100-911) or anti-CAP350 mouse antibody (CAP350m, bottom) and alpha-catenin antibodies. At right, high-magnification images of the boxed areas showing single labellings and merged images. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pbio.1002087) licensed under a CC-BY licence.

Immunoprecipitation: CEP350 Antibody [NB100-59811] - Detection of Human CAP350 on HeLa whole cell lysate using NB100-59811. CAP350 was also immunoprecipitated by rabbit anti-CAP350 antibody NB100-59810.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ICC/IF, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CEP350 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 3066 and 3116 of human centrosome-associated protein 350 using the numbering given in entry NP_055625.3 (GeneID 9857).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CEP350
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is not recommended for crude preparations but can be used to blot CAP350 in enriched sources. ICC/IF, WB reactivity reported in (PMID: 20861314).
• Theoretical MW: 351 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 25764135). Mouse reactivity reported in scientific literature (PMID: 28625565).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CEP350 Antibody

• CAP350GM133
• centrosomal protein 350kDa
• centrosome-associated protein 350
• Centrosome-associated protein of 350 kDa
• Cep350
• FLJ38282
• FLJ44058
• KIAA0480centrosome associated protein 350

Background

CAP350 (Centrosome-Associated Protein 350) has been shown to localize to the centrosome as well as to dots in the pericentrosomal area. CAP350 functions to stabilize and anchor microtubules to the centrosome and participates in the maintenance of a pericentrosomal Golgi ribbon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEP350 Antibody (NB100-59811)(7)

Publications using NB100-59811

• Tu HQ, Qin XH, Liu ZB et al. Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis Nat Commun Dec 11 2018 [PMID: 30538248] (ICC/IF, Human)
• Wang L, Failler M, Fu W, Dynlacht BD. A distal centriolar protein network controls organelle maturation and asymmetry. Nat Commun. Sep 26 2018 [PMID: 30258116] (ICC/IF, Human)
• kanie T, Abbott kL, MooneY NA et al. The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. Dev. Cell. Jul 10 2017 [PMID: 28625565] (ICC/IF, Mouse)
• Korzeniewski N, Cuevas R, Duensing A, Duensing S. Daughter centriole elongation is controlled by proteolysis. Mol Biol Cell 2010 Nov 15 [PMID: 20861314] (ICC/IF, WB, Human)
• Tischer T, Yang J, Barford D Negative feedback between the APC/C and Cep152 at the centrosome regulates mitotic spindle assembly BioRxiv Jan 1 2020 (WB, Human)
• Ryu H, Lee H, Lee J, et al. The molecular dynamics of subdistal appendages in multi-ciliated cells Nature communications Jan 27 2021 [PMID: 33504787] (IP, Mouse)
• Gavilan MP, Arjona M, Zurbano A et al. Alpha-catenin-Dependent Recruitment of the Centrosomal Protein CAP350 to Adherens Junctions Allows Epithelial Cells to Acquire a Columnar Shape. PLoS Biol. 2015 Mar 01 [PMID: 25764135] (WB, ICC/IF, Canine)

Bioinformatics

• Gene Symbol: CEP350
• Entrez:
  • 9857(Human)
• Uniprot:
  • Q5VT06(Human)