Centrin 1 Antibody (OTI1E1) - Azide and BSA Free

Western Blot: Centrin 1 Antibody (OTI1E1) - Azide and BSA Free [NBP2-70408] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Centrin 1.

Immunohistochemistry: Centrin 1 Antibody (OTI1E1) - Azide and BSA Free [NBP2-70408] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Centrin 1 Antibody (OTI1E1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Centrin 1 Antibody (OTI1E1) - Azide and BSA Free (NBP2-46253) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CETN1 (NP_004057) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CETN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 19.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Centrin 1 Antibody (OTI1E1) - Azide and BSA Free

• calcium binding protein
• Caltractin isoform 2
• caltractin
• CEN1centrin-1
• centrin, EF-hand protein, 1
• CETN
• EF-hand protein

Background

Centrin 1 is encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CETN1