CEACAM16 Antibody (2747B) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human CEACAM-16 (filled histogram) or irrelevant protein (open histogram) was stained with Rabbit Anti-Human CEACAM-16 Monoclonal Antibody (Catalog # MAB9885) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

CEACAM-16 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rabbit Anti-Human CEACAM-16 Monoclonal Antibody (Catalog # MAB9885) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 2747B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CEACAM16 Antibody (2747B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese Hamster Ovary cell line CHO-derived human CEACAM-16
  Met1-Gly425
  Accession # Q2WEN9
• Specificity: Detects human CEACAM-16 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM16 Antibody (2747B) [Unconjugated]

• carcinoembryonic antigen-related cell adhesion molecule 16
• CEACAM-16
• CEAL2
• DFNA4B

Background

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16 (CEACAM-16), or CEAL2, is part of the CEA protein family consisting of CEACAMs and the pregnancy‑specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Unique to the CEA family, CEACAM-16 is a secreted molecule lacking a recognizable transmembrane domain or GPI anchor (3). Mature human CEACAM-16 is 405 amino acids (aa), containing 2 IgC2-like domains and 2 IgV-like domains. CEACAM-16 is one of only five conserved CEACAMs among mice, rats, and humans (2). Mature human CEACAM-16 shares 90% and 89% aa identity with mouse and rat CEACAM‑16, respectively. Originally discovered as a biomarker for colorectal cancer (4), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5-7). CEACAM-16 is specifically expressed in the inner ear and has been shown to play a critical role in hearing. CEACAM-16 has been identified as a binding partner for alpha tectorin and specific mutations in CEACAM-16 have been linked to autosomal dominant nonsyndromic deafness (ADNSHL) (3, 8).

1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
2. Zebhauser R. et al. (2005) Genomics 86:566.
3. Zheng, J. et al. (2011) PNAS. 108(10):4218.
4. Gold P. and Freedman S.O. (1965) J Exp Med 122:467.
5. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
6. Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
7. Kuespert K et al. (2006) Curr Opin Cell Biol. 18(5):565.
8. Wang, H. et al. (2015) J Hum Genet. 60(3):119.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q2WEN9()