![Western Blot: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/CDKL5-Antibody-Western-Blot-NBP2-32157-img0001.jpg "Western Blot: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC-P, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 350 to 400 of human Cyclin-Dependent Kinase-Like 5 using the numbering given in entry NP_003150.1 (GeneID 6792). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CDKL5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Positive Control |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Diseases for CDKL5 Antibody (NBP2-32157)Discover more about diseases related to CDKL5 Antibody (NBP2-32157).
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PTMs for CDKL5 Antibody (NBP2-32157)Learn more about PTMs related to CDKL5 Antibody (NBP2-32157).
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| Gene Symbol | CDKL5 |
| Uniprot |
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![Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize2/CDKL5-Antibody-Immunohistochemistry-Paraffin-NBP2-32157-img0006.jpg "Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize2/CDKL5-Antibody-Immunohistochemistry-Paraffin-NBP2-32157-img0005.jpg "Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunoprecipitation: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for IP at 6 ug/mg lysate. CDKL5 was inefficiently immunoprecipitated by rabbit anti-CDKL5 antibody BL15151. For blotting immunoprecipitated CDKL5, NBP2-32157 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/CDKL5-Antibody-Immunoprecipitation-NBP2-32157-img0002.jpg "Immunoprecipitation: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for IP at 6 ug/mg lysate. CDKL5 was inefficiently immunoprecipitated by rabbit anti-CDKL5 antibody BL15151. For blotting immunoprecipitated CDKL5, NBP2-32157 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MeCP2 Antibody [NB100-56326] - analysis of MeCP2 in HeLa cell lysate using this antibody.](http://images.novusbio.com/fullsize2/MeCP2 Antibody-Western Blot-NB100-56326-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - MeCP2 antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize2/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56326-img0003.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - IHC analysis of a formalin fixed paraffin embedded section of mouse liver tissue using MeCP2 antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize2/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0007.jpg)
![Western Blot: SAE2 Antibody [NB120-22104] - Western bot analysis of SAE2 in HeLa cell lysate using NB120-22104 at 1.0 ug/ml.](http://images.novusbio.com/fullsize2/SAE2 Antibody-Western Blot-NB120-22104-img0001.jpg)
![Western Blot: FOXG1A/FOXG1B Antibody [NBP1-56594] - Jurkat cell lysate, Antibody Titration: 5.0-8.0ug/ml](http://images.novusbio.com/fullsize2/FOXG1A-FOXG1B-Antibody-Western-Blot-NBP1-56594-img0001.jpg)
![Immunohistochemistry-Paraffin: FOXG1A/FOXG1B Antibody [NBP1-56594] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize2/FOXG1A-FOXG1B-Antibody-Immunohistochemistry-Paraffin-NBP1-56594-img0004.jpg)
![Immunohistochemistry-Paraffin: FOXG1A/FOXG1B Antibody [NBP1-56594] - Human kidney.](http://images.novusbio.com/fullsize2/FOXG1A-FOXG1B-Antibody-Immunohistochemistry-Paraffin-NBP1-56594-img0005.jpg)
![Western Blot: Syntaxin-BP1 Antibody [NBP1-83139] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize2/Syntaxin-BP1-Antibody-Western-Blot-NBP1-83139-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Syntaxin-BP1 Antibody [NBP1-83139] - Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize2/Syntaxin-BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83139-img0007.jpg)
![Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining in human cerebral cortex and pancreas tissues using anti-STXBP1 antibody. Corresponding STXBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Syntaxin-BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83139-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Scn1a Antibody [NBP2-58876] - Staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize2/Scn1a Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58876-img0001.jpg)
![Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize2/DNMT1-Antibody-(60B1220.1)-Western-Blot-NB100-56519-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize2/DNMT1-Antibody-(60B1220.1)-Immunocytochemistry-Immunofluorescence-NB100-56519-img0006.jpg)
![Immunohistochemistry-Paraffin: DNMT1 Antibody (60B1220.1) [NB100-56519] - IHC-P validation of DNMT1 antibody (clone 60B1220.1) on formalin fixed and paraffin embedded tissue section of human hepatocellular carcinoma at 1:50 dilution.](http://images.novusbio.com/fullsize2/DNMT1-Antibody-(60B1220.1)-Immunohistochemistry-Paraffin-NB100-56519-img0007.jpg)
![Western Blot: Protocadherin-10 Antibody [NBP2-19719] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 5% SDS PAGE diluted at 1:50](http://images.novusbio.com/fullsize2/Protocadherin-10-Antibody-Western-Blot-NBP2-19719-img0004.jpg)
![Immunohistochemistry-Paraffin: Protocadherin-10 Antibody [NBP2-19719] - Analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Protocadherin-10-Antibody-Immunohistochemistry-Paraffin-NBP2-19719-img0003.jpg)
![Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize2/E6AP-UBE3A-Antibody-Western-Blot-NB500-240-img0002.jpg)
![Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.Lane 1: MBD5 transfected lysate(24.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/MBD5 Antibody (4A12-1B6)-Western Blot-H00055777-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of monoclonal antibody to MBD5 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/MBD5-Antibody-(4A12-1B6)-Immunocytochemistry-Immunofluorescence-H00055777-M01-img0003.jpg)
![Sandwich ELISA: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Detection limit for recombinant GST tagged MBD5 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/MBD5-Antibody-(4A12-1B6)-Sandwich-ELISA-H00055777-M01-img0004.jpg)
![Immunohistochemistry-Paraffin: NHS Antibody [NBP1-86329] - Staining of human liver shows strong cytoplasmic positivity( granular pattern) in hepatocytes.](http://images.novusbio.com/fullsize/NHS Antibody-Immunohistochemistry-Paraffin-NBP1-86329-img0002.jpg)
![Western Blot: SLC25A22 Antibody [NBP1-84880] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/SLC25A22-Antibody-Western-Blot-NBP1-84880-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SLC25A22 Antibody [NBP1-84880] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.](http://images.novusbio.com/fullsize2/SLC25A22 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84880-img0007.jpg)
![Immunohistochemistry-Paraffin: SLC25A22 Antibody [NBP1-84880] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells and neuropil.](http://images.novusbio.com/fullsize2/SLC25A22-Antibody-Immunohistochemistry-Paraffin-NBP1-84880-img0006.jpg)
![Western Blot: PNK Antibody [NBP1-87257] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize2/PNK-Antibody-Western-Blot-NBP1-87257-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PNK Antibody [NBP1-87257] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize2/PNK Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87257-img0011.jpg)
![Immunohistochemistry-Paraffin: PNK Antibody [NBP1-87257] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize2/PNK-Antibody-Immunohistochemistry-Paraffin-NBP1-87257-img0001.jpg)
![Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize2/SGSM3-Antibody-Western-Blot-NBP2-13304-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize2/SGSM3 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13304-img0005.jpg)
![Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/SGSM3-Antibody-Immunohistochemistry-Paraffin-NBP2-13304-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0005.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.")