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CDKL5 Antibody

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Western Blot: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for WB at 0.4 ug/ml. Detection: ...read more
Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. ...read more
Immunohistochemistry-Paraffin: CDKL5 Antibody [NBP2-32157] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CDKL5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. ...read more
Immunoprecipitation: CDKL5 Antibody [NBP2-32157] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDKL5 antibody NBP2-32157 used for IP at 6 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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CDKL5 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 350 to 400 of human Cyclin-Dependent Kinase-Like 5 using the numbering given in entry NP_003150.1 (GeneID 6792).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDKL5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDKL5 Antibody

  • CDKL5
  • cyclin-dependent kinase-like 5
  • serine/threonine kinase 9
  • serine/threonine-protein kinase 9
  • STK9

Background

CDKL5 - cyclin-dependent kinase-like 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - This antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Tissue section of mouse pancreas using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in majority of the cells from acini, ducts and Islets of Langerhans in the section.
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Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.
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Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
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Bioinformatics

Gene Symbol CDKL5
Uniprot