CCDC28B Overexpression Lysate (Native)

Images

 
Western Blot: CCDC28B Overexpression Lysate (Adult Normal) [NBL1-08791] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCDC28B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CCDC28B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203631 and based on accession number NM_024296. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens coiled-coil domain containing 28B (CCDC28B), mRNA.
Gene
CCDC28B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CCDC28B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CCDC28B Overexpression Lysate (Native)

  • coiled-coil domain containing 28B
  • coiled-coil domain-containing protein 28B
  • MGC1203
  • MGC16441
  • RP4-622L5.5

Background

The product of this gene localizes to centrosomes and basal bodies. It interacts and colocalizes with several proteins associated with Bardet-Biedl syndrome (BBS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34106
Species: Hu
Applications: IHC, IHC-P
NBP1-91695
Species: Hu
Applications: IHC, IHC-P
NBP1-88688
Species: Hu
Applications: IHC, IHC-P
AF482
Species: Mu
Applications: WB, IHC
NBP1-80848
Species: Hu
Applications: IHC, IHC-P
NBP1-86248
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88690
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, ICC
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-97823
Species: Hu
Applications: WB, ICC/IF, IP
NBP2-33404
Species: Hu
Applications: IHC, IHC-P
NB200-118
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-33581
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP1-88927
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBL1-08791
Species: Hu
Applications: WB

Publications for CCDC28B Lysate (NBL1-08791) (0)

There are no publications for CCDC28B Lysate (NBL1-08791).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CCDC28B Lysate (NBL1-08791) (0)

There are no reviews for CCDC28B Lysate (NBL1-08791). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCDC28B Lysate (NBL1-08791) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CCDC28B Products

Bioinformatics Tool for CCDC28B Lysate (NBL1-08791)

Discover related pathways, diseases and genes to CCDC28B Lysate (NBL1-08791). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CCDC28B Lysate (NBL1-08791)

Discover more about diseases related to CCDC28B Lysate (NBL1-08791).

Blogs on CCDC28B

There are no specific blogs for CCDC28B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CCDC28B Antibody
NBP2-15747

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCDC28B Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CCDC28B