Caspase-6 Antibody

Product Discontinued

Caspase-6 Antibody Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Caspase-6. The immunogen is located within the first 50 amino acids of Caspase-6.
• Specificity: Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody can be used for the detection of caspase-6 by Western blot at 0.5 mg/ml. Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Caspase-6 Antibody

• Apoptotic protease Mch-2
• CASP6
• CASP-6
• caspase 6, apoptosis-related cysteine peptidase
• caspase 6, apoptosis-related cysteine protease
• Caspase6
• Caspase-6
• EC 3.4.22
• Mch2
• MCH2EC 3.4.22.59

Background

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-6 is an executioner caspase that was idientifed based on its homology with human caspases 2 and 3 as well as the C. elegans cell death protein CED-3. It possesses two isoforms, of which only the longer form possesses protease activity. Caspase-6 is highly expressed in adult brain and may play a role in several neuronal pathologies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP6
• Uniprot:
  • NP_001217(Human)