Carbohydrate Sulfotransferase 4/CHST4 Antibody (595223) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: IP, B/N
• Clone: 595223
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Carbohydrate Sulfotransferase 4/CHST4 Antibody (595223) [Alexa Fluor® 594] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Sulfotransferase 4/CHST4
  His29-His386
  Accession # Q8NCG5
• Specificity: Detects human Carbohydrate Sulfotransferase 4/CHST4 is direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHST1, 2, 5, 6, 10, 14, recombinant mouse CHST3, 4, and 7 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Neutralization

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 4/CHST4 Antibody (595223) [Alexa Fluor® 594]

• carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
• Carbohydrate Sulfotransferase 4
• CHST4
• EC 2.8.2
• EC 2.8.2.-
• EC 2.8.2.17
• EC 2.8.2.21
• galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3
• Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3
• GlcNAc6ST2
• GlcNAc6ST-2
• gn6st-2
• GST3
• GST-3
• HECGLCNAC6ST
• HEC-GlcNAc6ST
• HEC-GLCNAC-6-ST
• High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
• L-selectin ligand sulfotransferase
• LSST
• LSSTcarbohydrate sulfotransferase 4
• N-acetylglucosamine 6-O-sulfotransferase 2

Background

The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also known as high endothelial cells N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L‑selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L‑selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O‑glycans, but also has activity toward core 3 type of O‑glycan (5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.