CaMKII beta/gamma Antibody (SU03-57)

Western Blot: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Western blot analysis of CaMKII beta/gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. G

Immunocytochemistry/Immunofluorescence: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Staining CaMKII in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Analysis of paraffin-embedded rat cerebellum tissue using anti-CaMKII antibody. Counter stained with hematoxylin.

Western Blot: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Analysis of CaMKII on different lysates using anti-CaMKII antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: PC-12 Lane 3: SHG-44

Immunocytochemistry/Immunofluorescence: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Staining CaMKII in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Staining CaMKII in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Analysis of paraffin-embedded mouse brain tissue using anti-CaMKII antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKII antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CaMKII beta/gamma Antibody (SU03-57) [NBP2-67476] - Analysis of paraffin-embedded rat brain tissue using anti-CaMKII antibody. Counter stained with hematoxylin.

Product Discontinued

CaMKII beta/gamma Antibody (SU03-57) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CaMKII beta/gamma aa 201-250 / 478. (SwissProt: Q13554 Human; SwissProt: Q13555 Human; SwissProt: Q13557 Human; SwissProt: Q9UQM7 Human; SwissProt: P11798 Mouse; SwissProt: P28652 Mouse; SwissProt: Q6PHZ2 Mouse; SwissProt: Q923T9 Mouse; SwissProt: P08413 Rat; SwissProt: P11275 Rat; SwissProt: P11730 Rat; SwissProt: P15791 Rat)
• Localization: Cytoplasm, Sarcoplasmic reticulum membrane, Cell membrane, Cell junction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAMK2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CaMKII beta/gamma Antibody (SU03-57)

• calcium/calmodulin-dependent protein kinase (CaM kinase) II beta
• calcium/calmodulin-dependent protein kinase II beta
• calcium/calmodulin-dependent protein kinase type II beta chain
• calcium/calmodulin-dependent protein kinase type II subunit beta
• CaM kinase II beta subunit
• CaM kinase II subunit beta
• CAM2CAMK2
• CAMKB
• CaMK-II subunit beta
• CaM-kinase II beta chain
• EC 2.7.11
• EC 2.7.11.17
• MGC29528
• proline rich calmodulin-dependent protein kinase

Background

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated alpha, beta, gamma and delta, which may or may not be coexpressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK2B