Recombinant Human CACNB3 GST (N-Term) Protein

SDS-Page: Recombinant Human CACNB3 Protein [H00000784-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP

Recombinant Human CACNB3 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-484 of Human CACNB3
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MYDDSYVPGFEDSEAGSADSYTSRPSLDSDVSLEEDRESARREVESQAQQQLERAKHKPVAFAVRTNVSYCGVLDEECPVQGSGVNFEAKDFLHIKEKYSNDWWIGRLVKEGGDIAFIPSPQRLESIRLKQEQKARRSGNPSSLSDIGNRRSPPPSLAKQKQKQAEHVPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADLSLAKRSVLNNPGKRTIIERSSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHPAQLAKTSLAPIIVFVKVSSPKVLQRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVILDENQLEDACEHLAEYLEVYWRATHHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQRSSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWPKDSY

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: CACNB3
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 80.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human CACNB3 GST (N-Term) Protein

• CAB3
• CACNLB3
• Calcium channel voltage-dependent subunit beta 3
• calcium channel, voltage-dependent, beta 3 subunit
• voltage-dependent L-type calcium channel subunit beta-3

Background

The CACNB3 gene encodes for a proteins that are long voltage dependent L-type calcium channel subunit beta-3 gene that aids in regulating surface expression, calcium transport, regulation of transcription factors, and gating of calcium channels. Isoform 3A is 484 amino acids in length with a mass of around 54 kDA while isoform 3B is 447 amino acids long at around 50 kDA. Increasingly, research has investigated the role of the CACNB3 gene in various diseases such as neuronitis, neuropathy, canavan disease, and lambert-eaton myasthenic syndrome. This gene plays a role in many pathways such as CREB transcription, the Celecoxib pathway, MAPK signaling pathway, and the Myometrial Relaxation and Contraction pathways and is known to interact with various other genes such as: CACNA1C, CACNA1B, SYT1, FASLG, and YWHAB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CACNB3