CACNB3 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

CACNB3 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CACNB3.

  Source: E. coli

  Amino Acid Sequence: SIRLKQEQKARRSGNPSSLSDIGNRRS

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: CACNB3
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-56436.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for CACNB3 Recombinant Protein Antigen

• CAB3
• CACNLB3
• Calcium channel voltage-dependent subunit beta 3
• calcium channel, voltage-dependent, beta 3 subunit
• voltage-dependent L-type calcium channel subunit beta-3

Background

The CACNB3 gene encodes for a proteins that are long voltage dependent L-type calcium channel subunit beta-3 gene that aids in regulating surface expression, calcium transport, regulation of transcription factors, and gating of calcium channels. Isoform 3A is 484 amino acids in length with a mass of around 54 kDA while isoform 3B is 447 amino acids long at around 50 kDA. Increasingly, research has investigated the role of the CACNB3 gene in various diseases such as neuronitis, neuropathy, canavan disease, and lambert-eaton myasthenic syndrome. This gene plays a role in many pathways such as CREB transcription, the Celecoxib pathway, MAPK signaling pathway, and the Myometrial Relaxation and Contraction pathways and is known to interact with various other genes such as: CACNA1C, CACNA1B, SYT1, FASLG, and YWHAB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CACNB3