CACNB3 Antibody

Western Blot: CACNB3 Antibody [NBP2-76916] - Western blot analysis of CACNB3 on different lysates using anti-CACNB3 antibody at 1/500 dilution.Positive control:Lane1: Rat brain tissueLane2: SKOV-3

Immunohistochemistry: CACNB3 Antibody [NBP2-76916] - Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-CACNB3 antibody. Counter stained with hematoxylin.

Flow Cytometry: CACNB3 Antibody [NBP2-76916] - Flow cytometric analysis of SKOV-3 cells with CACNB3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: CACNB3 Antibody [NBP2-76916] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNB3 antibody. Counter stained with hematoxylin.

Immunohistochemistry: CACNB3 Antibody [NBP2-76916] - Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CACNB3 antibody. Counter stained with hematoxylin.

Product Discontinued

CACNB3 Antibody Summary

• Immunogen: Synthetic peptide within Human CACNB3 aa 435-484 / 484. (SwissProt: P54284 Human; SwissProt: P54285 Mouse; SwissProt: P54287 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CACNB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CACNB3 Antibody

• CAB3
• CACNLB3
• Calcium channel voltage-dependent subunit beta 3
• calcium channel, voltage-dependent, beta 3 subunit
• voltage-dependent L-type calcium channel subunit beta-3

Background

The CACNB3 gene encodes for a proteins that are long voltage dependent L-type calcium channel subunit beta-3 gene that aids in regulating surface expression, calcium transport, regulation of transcription factors, and gating of calcium channels. Isoform 3A is 484 amino acids in length with a mass of around 54 kDA while isoform 3B is 447 amino acids long at around 50 kDA. Increasingly, research has investigated the role of the CACNB3 gene in various diseases such as neuronitis, neuropathy, canavan disease, and lambert-eaton myasthenic syndrome. This gene plays a role in many pathways such as CREB transcription, the Celecoxib pathway, MAPK signaling pathway, and the Myometrial Relaxation and Contraction pathways and is known to interact with various other genes such as: CACNA1C, CACNA1B, SYT1, FASLG, and YWHAB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNB3