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BRN4 Recombinant Protein Antigen

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BRN4 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human POU3F4.

Source: E.coli

Amino Acid Sequence: RSPHVAHHSPHTNHPNAWGASPAPNPSITSSGQPLNVYSQPGFTVSGMLEHGGLTPPPAAASAQSLHPVLREPPDHGELGSHHCQDH
Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
POU3F4
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This peptide is useful as a blocking peptide for NBP1-89934.
Protein was purified by IMAC chromatography. The expected concentration is greater than 0.5 mg/ml. This product is produced on demand, estimated shipping date is 4 weeks after the order is placed.

For further blocking peptide related information and a protocol, click here.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Alternate Names for BRN4 Recombinant Protein Antigen

  • brain-specific homeobox/POU domain protein 4
  • octamer-binding transcription factor 9
  • OTF9
  • POU class 3 homeobox 4
  • POU domain, class 3, transcription factor 4

Background

BRN4 encodes a member of the POU-III class of neural transcription factors. This gene may play a role in the mediation of epigenetic signals which induce striatal neuron-precursor differentiation. Mutations have been associated with X chromosome-linked nonsyndromic mixed deafness. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Frozen: POU3F2/OCT7 Antibody [NBP2-21585] - Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody ( diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [1710] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - Mouse brain. Brn2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - Rat brain. Brn2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Species: Pm, Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
POU3F3 Antibody
NBP1-49872
Western Blot: POU3F3 Antibody [NBP1-49872] - Staining of Mouse Spinal Cord (A) and Brain (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: POU3F3 Antibody [NBP1-49872] - Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: POU3F3 Antibody [NBP1-49872] - Flow cytometric analysis of paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(1 Review)
(5 Publications)
Species: Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA, WB
OCT6 Antibody
NBP3-13349
Western Blot: OCT6 Antibody [NBP3-13349] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with OCT6 antibody (NBP3-13349) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:500. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Connexin 26/GJB2
Antibody - BSA Free
NBP2-41304
Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Analysis of Connexin 26/GJB2 in human colon tissue lysate with Connexin 26/GJB2 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/Immunofluorescence: Connexin 26/GJB2 Antibody [NBP2-41304] - Cx26 distribution at level of the cardiomyocyte cytoplasm. Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R(alpha)Cx26-cl and M(alpha)Cx43. Scale bar: 20um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY licence.
Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Positive and negative tissue controls for Cx26. Representative images of immunohistochemical analysis for Cx26 on rat liver and skeletal muscle sections by R(alpha)Cx26-cl antibody. Cx26 positive brown punctate staining of hepatocyte plasma membrane (arrows) and cytoplasm (arrowheads) is shown in liver. By contrast, Cx26 staining is absent in skeletal muscle. Negative controls are showen in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY licence.
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Applications: ELISA, ICC/IF, IHC, IHC-P, WB
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Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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PDX-1/IPF1
Antibody [Unconjugate
AF2419
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TBX1 Antibody
(OTI1C2)
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Neurogenin-3
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Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of GJB3 expression in transfected 293T cell line by GJB3 monoclonal antibody (M01), clone 3B4-1B3.Lane 1: GJB3 transfected lysate(31 KDa).Lane 2: Non-transfected lysate.
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Insulin Antibody
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OCT1 Antibody
NBP2-24677
Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-24677] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-OCT1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-24677] - Analysis of OCT-1 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
Western Blot: OCT1 Antibody [NBP2-24677] - Western blot analysis of OCT-1 in human HL60 cell lysate using NBP2-24677 at 4 ug/ml.
Species: Ca, Eq, Hu, Pm
Applications: ICC/IF, IHC, IHC-P, WB
Pax6 Antibody
NB300-750
Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract
Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.
Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract
(4 Reviews)
(5 Publications)
Species: Fi, Hu, Pm, Mu, Pm, Rt, Sh
Applications: ICC/IF, IHC, IP, WB
CHM Antibody
NBP1-84954
Immunohistochemistry-Paraffin: CHM Antibody [NBP1-84954] - Staining of human cerebellum shows strong positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: CHM Antibody [NBP1-84954] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CHM Antibody [NBP1-84954] - Staining of human cerebral cortex shows moderate positivity in neurons.
Species: Hu
Applications: IHC, IHC-P
PRPS1 Antibody
NBP2-13813
Immunocytochemistry/Immunofluorescence: PRPS1 Antibody [NBP2-13813] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PRPS1 Antibody [NBP2-13813] - Staining of human endometrium shows moderate nuclear positivity in stromal cells.
Immunohistochemistry-Paraffin: PRPS1 Antibody [NBP2-13813] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Pancreatic
Polypeptide/PP Antibo
NB100-1793
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
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Proprotein
Convertase 1/PCSK1 An ...
H00005122-M02
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Immunohistochemistry-Paraffin: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Analysis of monoclonal antibody to PCSK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Sandwich ELISA: Proprotein Convertase 1/PCSK1 Antibody (3D2) [H00005122-M02] - Detection limit for recombinant GST tagged PCSK1 is approximately 0.03ng/ml as a capture antibody.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
Amylin Antibody
NBP2-33680
Immunohistochemistry: Amylin Antibody [NBP2-33680] - Staining of human pancreas shows distinct cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: Amylin Antibody [NBP2-33680] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Amylin Antibody [NBP2-33680] - Staining in human pancreas and skeletal muscle tissues . Corresponding IAPP RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
PDHX Antibody
NBP1-84079
Immunohistochemistry-Paraffin: PDHX Antibody [NBP1-84079] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: PDHX Antibody [NBP1-84079] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: PDHX Antibody [NBP1-84079] - Staining in human heart muscle and pancreas tissues using anti-PDHX antibody. Corresponding PDHX RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P, WB

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Bioinformatics

Gene Symbol POU3F4