Biotinidase/BTD Antibody

Western Blot: Biotinidase/BTD Antibody [NBP2-26186] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Biotinidase/BTD Antibody Summary

• Description: Novus Biologicals Goat Biotinidase/BTD Antibody (NBP2-26186) is a polyclonal antibody validated for use in WB and ELISA. Anti-Biotinidase/BTD Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence EKDAQEVHCDEATK corresponding to internal region according to NP_000051.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BTD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human liver lysates (calculated MW of 61.1 kDa band according to NP_000051.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Biotinidase/BTD Antibody

• Biotinase
• Biotinidase
• BTD
• EC 3.5.1
• EC 3.5.1.12

Background

Biotinidase functions to recycle biotin in the body by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. Biotinidase has also been shown to have biotinyl-transferase activity. Defects in the biotinidase gene cause multiple carboxylase deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Biotinidase/BTD Antibody (NBP2-26186)(1)

Publication using NBP2-26186

• Cowan TM, Blitzer MG, Wolf B. Technical standards and guidelines for the diagnosis of biotinidase deficiency. Genet Med 2010-07-01 [PMID: 20539236]

Bioinformatics

• Gene Symbol: BTD
• Entrez:
  • 686(Human)