Beta Dystroglycan Antibody (EP2200Y)

Product Discontinued

Beta Dystroglycan Antibody (EP2200Y) Summary

• Immunogen: A synthetic peptide corresponding o residues near the C-terminus of human Beta-Dystroglycan was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DAG1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: This product is useful for: Western Blot. In Western blot this antibody detects a band at approximately 43kDa.

Reactivity Notes

Reacts with: Human Does not react with Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Beta Dystroglycan Antibody (EP2200Y)

• A3a
• AGRNR
• DAG156DAG
• dystroglycan 1 (dystrophin-associated glycoprotein 1)
• dystroglycan
• Dystrophin-associated glycoprotein 1

Background

The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAG1
• Entrez:
  • 1605(Human)
• Uniprot:
  • Q14118(Human)