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AXUD1 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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AXUD1 Antibody Summary

Immunogen
AXUD1 protein
Specificity
Reacts with the human AXUD1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSRNP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry-frozen and paraffin.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
AXUD1 Overexpression Lysate
Publications
Read Publication using NB200-144.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AXUD1 Antibody

  • AXIN1 up-regulated 1
  • Axin-1 up-regulated gene 1 protein
  • AXUD1DKFZp566F164
  • CSRNP-1
  • cysteine/serine-rich nuclear protein 1
  • cysteine-serine-rich nuclear protein 1
  • FAM130B
  • Protein URAX1
  • TAIP3
  • TAIP-3TGF-beta-induced apoptosis protein 3
  • TGF-beta induced apoptosis protein 3
  • URAX1

Background

Axin, an important regulator of beta-catenin, is frequently mutated in human hepatocellular carcinomas (HCCs), and transduction of the wild-type Axin gene (AXIN1) induces apoptosis in HCC cells as well as in colon cancer cells. A novel gene, AXUD1 (AXIN 1 up-regulated), has enhanced expression in response to exogenously expressed AXIN1. It is expressed in all human tissues examined, most abundantly in lung, placenta, skeletal muscle, pancreas and leukocytes. Data suggests that AXUD1 may have a tumor-suprressor function in those organs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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IL-2 [Unconjugated]
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1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
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COM1/p8 Antibody
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Western Blot: COM1/p8 Antibody [NBP1-98280] - 293T Cell Lysate 1.0ug/ml, Gel Concentration: 10-20%
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Species: Hu
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Sonic Hedgehog/Shh
[Unconjugated]
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1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029  is a matrix-associated artifact of the MALDI-TOF.
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 μg/mL.
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beta-Catenin
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Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta-tubulin (55 kDa). Total and active beta-catenin correspond to 92 kDa. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0112388), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).
(4 Reviews)
(12 Publications)
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
CSRNP3 Antibody
NBP1-83933
Western Blot: CSRNP3 Antibody [NBP1-83933] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: CSRNP3 Antibody [NBP1-83933] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
Immunohistochemistry-Paraffin: CSRNP3 Antibody [NBP1-83933] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
Species: Hu, Mu, Rt
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GADD34 Antibody
(OTI3D12)
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Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/Immunofluorescence: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD34.
Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.
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Species: Hu, Mu, Rt, Ca, Pm
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(OTI3G8)
NBP2-02340
Western Blot: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT1 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT1.
Immunohistochemistry-Paraffin: IFIT1 Antibody (OTI3G8) [NBP2-02340] - Staining of paraffin-embedded Human liver tissue using anti-IFIT1 mouse monoclonal antibody.
Species: Hu, Mu, Rt, Pm
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Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood. PBMC were stained in a 2 color flow test, with CD14 PE version of this antibody and 1 ug of either isotype control (left) or TLR4-Alexa Fluor 647 (right). PPI negative, CD14+ cells were gated for analysis.
Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (http://www.jeccr.com/content/33/1/45), licensed under a CC-BY licence.
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AXUD1 Antibody
NB200-144
AXUD1 Antibody
Species: Hu
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P

Publications for AXUD1 Antibody (NB200-144)(1)


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Control Lysate(s)

AXUD1 Overexpression Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CSRNP1
Entrez