Recombinant Human ASF1b His Protein

SDS-Page: Recombinant Human ASF1b Protein [NBP1-98888] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human ASF1b His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-202 of Human ASF1b
  
  

  Source: E.coli

  Amino Acid Sequence: MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILEHHHHHH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ASF1B
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 23.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human ASF1b His Protein

• ASF1 silencing function 1 homolog B (S. cerevisiae)
• CCG1-interacting factor A-II
• CIA-II
• FLJ10604
• hAsf1
• hAsf1b
• hCIA-II
• histone chaperone ASF1B
• silencing function protein 1 homolog B

Background

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 protein in yeast. This protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. Recombinant human ASF1B protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ASF1B