ARL13B Antibody (N295B/66) - Chimeric - BSA Free

Representative section of formalin fixed, paraffin-embedded rat brain lateral ventricle region showing cells stained by NBP3-52344 (visualized in green) at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the ciliated cells surrounding the lateral ventricle. Magnification 400X.

Representative section of formalin fixed, paraffin-embedded rat brain lateral ventricle region showing cells stained by NBP3-52344 (visualized in green) at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the ciliated cells surrounding the lateral ventricle. Magnification 400X.

• Reactivity: Hu, Mu, Rt, Pm, Ze
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: N295B/66
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ARL13B Antibody (N295B/66) - Chimeric - BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N295B/66 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N295B/66 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 208-427 (C-terminus) of mouse ARL13B (Uniprot#: Q640N2) produced recombinantly in E. Coli
• Specificity: Does not cross-react with Arl13a (based on KO validation results)
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: ARL13B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Western Blot 1:1000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for ARL13B Antibody (N295B/66) - Chimeric - BSA Free

• ADP-ribosylation factor-like 13B
• ADP-ribosylation factor-like 2-like 1
• ADP-ribosylation factor-like protein 13B
• ADP-ribosylation factor-like protein 2-like 1
• ARL2L1MGC120611
• ARL2-like protein 1
• DKFZp686E2075
• DKFZp686L2472
• DKFZp761H079
• JBTS8DKFZp686M2074
• MGC120612

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARL13B