ARFGAP3 Antibody

Western Blot: ARFGAP3 Antibody [NB100-843] - (2ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Mu, Hu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARFGAP3 Antibody Summary

• Immunogen: Peptide with sequence C-NGVVTSIQDRYGS corresponding to C-Terminus according to NP_055385.3, NP_001135765.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_055385.3; NP_001135765.1).
• Predicted Species: Human (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARFGAP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 55-60 kDa band observed in mouse brain extracts.
• Control:

  ARFGAP3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARFGAP3 Antibody

• ADP-ribosylation factor GTPase activating protein 1
• ADP-ribosylation factor GTPase activating protein 3
• ADP-ribosylation factor GTPase-activating protein 3
• ARF GAP 3
• ARFGAP1
• FLJ45618

Background

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which is thought to interact with ADP-ribosylation factor 1 (ARF1). The encoded protein likely promotes hydrolysis of ARF1-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARFGAP3 Antibody (NB100-843)(1)

Publication using NB100-843

• Liu X, Zhang C, Xing G et al. Functional characterization of novel human ARFGAP3. FEBS Lett 2001-02-09 [PMID: 11172815]

Bioinformatics

• Gene Symbol: ARFGAP3
• Entrez:
  • 55738(Human)
  • 66251(Mouse)
• Uniprot:
  • NP_055385.(Human)