ARAP3 Overexpression Lysate

Images

 
Western Blot: ARAP3 Overexpression Lysate (Adult Normal) [NBP2-06429] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ARAP3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ARAP3 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_071926. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ARAP3, mRNA.
Gene
ARAP3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
169.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ARAP3 Overexpression Lysate

  • Arf and Rho GAP adapter protein 3
  • arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
  • ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
  • ARF-GAP, RHO-GAP, ankyrin repeat and plekstrin homology domains-containingprotein 3
  • centaurin, delta 3
  • centaurin-delta-3
  • CENTD3
  • cnt-d3
  • DRAG1
  • FLJ21065
  • phosphoinositide binding protein
  • PtdIns(34,5)P3-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67165
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB100-91273
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-55408
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46411
Species: Hu
Applications: WB, IHC
NBP2-38535
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-47602
Species: Hu, Mu, Bv, Vb
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-58359
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP
Applications: WB, IHC, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP, KD
NBP2-75641
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
H00003636-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-1343
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-P, PEP-ELISA
NBP2-67627
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84957
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-65538
Species: Hu

Publications for ARAP3 Lysate (NBP2-06429) (0)

There are no publications for ARAP3 Lysate (NBP2-06429).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ARAP3 Lysate (NBP2-06429) (0)

There are no reviews for ARAP3 Lysate (NBP2-06429). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ARAP3 Lysate (NBP2-06429) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBP2-06429

Additional ARAP3 Products

Bioinformatics Tool for ARAP3 Lysate (NBP2-06429)

Discover related pathways, diseases and genes to ARAP3 Lysate (NBP2-06429). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ARAP3 Lysate (NBP2-06429)

Discover more about diseases related to ARAP3 Lysate (NBP2-06429).
 

Pathways for ARAP3 Lysate (NBP2-06429)

View related products by pathway.

PTMs for ARAP3 Lysate (NBP2-06429)

Learn more about PTMs related to ARAP3 Lysate (NBP2-06429).

Blogs on ARAP3

There are no specific blogs for ARAP3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ARAP3 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ARAP3