AP3B1 Antibody

Flow Cytometry: AP3B1 Antibody [NBP2-61999] - Paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Product Discontinued

AP3B1 Antibody Summary

• Immunogen: Peptide with sequence SSNSFPYNEQSGG-C, from the N Terminus of the protein sequence according to NP_003655.
• Epitope: SSNSFPYNEQSGG-C
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AP3B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Peptide ELISA Detection Limit 1:64000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for AP3B1 Antibody

• Adapter-related protein complex 3 subunit beta-1
• Adaptor protein complex AP-3 subunit beta-1
• adaptor-related protein complex 3, beta 1 subunit
• ADTB3
• ADTB3APE
• AP-3 complex subunit beta-1
• beta-3A-adaptin
• beta3A-adaptin
• Clathrin assembly protein complex 3 beta-1 large chain
• HPS
• HPS2AP-3 complex beta-3A subunit

Background

AP3B1, also known as Adaptor protein 3 beta 3A, is specific for Adaptor Protein 3, one of four adaptor proteins interactive with endosomal Toll-like Receptors that mediate TLR trafficking and signaling cascades through the ER-endosomal systemIt has been shown specifically for TLR3, 7 and 9, all important innate immune anti-viral sensors, that interaction with UNC93B through AP-3 (AP3B1) allows loaded TLR transport from the endoplasmic reticulum to endolysosmes (1-4).The adaptor proteins AP-1, AP-2, AP-3, and AP-4 are utilized by UNC93B in trafficking and signaling pathways of TLRsThe AP3B1 antibody could be used to confirm AP-3 location on the endosomal membrane (4)AP-3 is critical for allowing TLR9 binding and delivery of its bound ligand, such as viral or parasitic RNA PAMP, from the ER to endolysomes which occurs normally in plasmacytoid dendritic cells.In AP-3 deficient cells, CpG -TLR9 activation of IFN expression does not occurThis dysregualtion is thought to be due to a failure of TLR9 engagement of IRF7 and TLR9 trafficking into the endolysosomeAP3B1 monitoring and AP3B1 quantitation therefore could serve as a model to examine the related TLR9 signaling, trafficking and activation pathway network.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP3B1