Amiloride-sensitive cation channel 3 Antibody

Immunocytochemistry/Immunofluorescence: Amiloride-sensitive cation channel 3 Antibody [NB100-2067] - ASIC3 expression in normal and CF human lung tissues by immunofluorescence microscopy. Normal (top panels A-C) and CF human lung sections (bottom panels D-F) were labeled with a specific antibody against ASIC3 (green channel) and the DNA-selective Hoechst dye (blue channel). A and D, normal and CF human lung sections imaged following incubation with the mixture of anti-ASIC3 antibody and immunopeptide. Shown are normal (B) and CF (E) alveolar structures at a small magnification. ASIC3 expression was detected in both types I and II alveolar cells. Shown are normal (C) and CF (F) alveolar structures at a large magnification. Scale bars equal 10 um in A, B, D, and E and 20 um in C and F.

Immunohistochemistry: Amiloride-sensitive cation channel 3 Antibody [NB100-2067] - ASIC3 labeling is done on cryostat sections of rat DRG using HRP-DAB detection (brown color).

Product Discontinued

Amiloride-sensitive cation channel 3 Antibody Summary

• Immunogen: Synthetic peptide: TASLDPDDFDPEPSDPLGSP, corresponding to amino acids 285-304 of extracellular domain of Rat ASIC3.
• Localization: Plasma membrane
• Specificity: ASIC3
• Isotype: IgG
• Clonality: Polyclonal
• Host: Guinea Pig
• Gene: ASIC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Reacts with Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Amiloride-sensitive cation channel 3 Antibody

• ACCN3
• Acid-sensing ion channel 3
• Amiloride-sensitive cation channel 3
• amiloride-sensitive cation channel 3, testis
• ASIC3
• ASIC3hTNaC1
• DRASIC
• hASIC3
• modulatory subunit of ASIC2a
• Neuronal amiloride-sensitive cation channel 3
• proton-gated cation channel subunit
• SLNAC1
• Testis sodium channel 1
• TNAC1

Background

The Amiloride-sensitive cation channel 3 gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASIC3
• Entrez:
  • 286920(Rat)