alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human a-N-terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-N-terminal Methyltransferase 1A/ METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha-N-terminal Methyltransferase 1A/METTL11A Antibody Summary

• Immunogen: E. coli-derived recombinant human alpha -N-terminal Methyltransferase 1A/METTL11A
  Thr2-Arg223
  Accession # Q9BV86
• Specificity: Detects human alpha -N-terminal Methyltransferase 1A/METTL11A in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NTMT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-N-terminal Methyltransferase 1A/METTL11A Antibody

• AD-003
• alpha N-terminal protein methyltransferase 1A
• C9orf32
• chromosome 9 open reading frame 32
• EC 2.1.1.-
• methyltransferase like 11A
• Methyltransferase-like protein 11A
• METTL11A
• NRMT
• N-terminal RCC1 methyltransferase
• NTM1A
• NTMT1
• X-Pro-Lys N-terminal protein methyltransferase 1A

Background

METTL11A, also named N‑terminal RCC1 methyltransferase (NRMT), is an alpha‑N‑methyltransferase that methylates the N‑terminal amino group of target proteins containing the N‑terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N‑terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman‑like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide‑exchange factor) is the first protein for which any biological function of alpha‑N‑methylation by NRMT has been identified (3, 4). The multi‑spindle phenotype associated with either NRMT knockdown or methylation‑defective RCC1 mutants demonstrated the importance of alpha‑N‑methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers.

1. Webb, K. J. et al. (2010) Biochemistry 49:5225.
2. Christine, S. E. et al. (2010) Nature 466:1125.
3. Chen, T. et al. (2007) Nature Cell Biol. 9:596.
4. Hao, Y. et al. (2008) J. Cell. Biol. 182:827.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NTMT1
• Uniprot:
  • Q9BV86()