alpha-N-terminal Methyltransferase 1A/METTL11A Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

alpha-N-terminal Methyltransferase 1A/METTL11A Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human α-N-terminal Methyltransferase 1A/METTL11A
  Thr2-Arg223
  Accession # Q9BV86
• Specificity: Detects human alpha -N-terminal Methyltransferase 1A/METTL11A in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-N-terminal Methyltransferase 1A/METTL11A Antibody [Alexa Fluor® 488]

• AD-003
• alpha N-terminal protein methyltransferase 1A
• C9orf32
• chromosome 9 open reading frame 32
• EC 2.1.1.-
• methyltransferase like 11A
• Methyltransferase-like protein 11A
• METTL11A
• NRMT
• N-terminal RCC1 methyltransferase
• NTM1A
• NTMT1
• X-Pro-Lys N-terminal protein methyltransferase 1A

Background

METTL11A, also named N‑terminal RCC1 methyltransferase (NRMT), is an alpha‑N‑methyltransferase that methylates the N‑terminal amino group of target proteins containing the N‑terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N‑terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman‑like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide‑exchange factor) is the first protein for which any biological function of alpha‑N‑methylation by NRMT has been identified (3, 4). The multi‑spindle phenotype associated with either NRMT knockdown or methylation‑defective RCC1 mutants demonstrated the importance of alpha‑N‑methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.