ADAM7 Antibody (4D1)

Western Blot: ADAM7 Antibody (4D1) [NBP2-75396] - Analysis of Gp83 on SH-SY-5Y cells lysates using anti-Gp83 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ADAM7 Antibody (4D1) [NBP2-75396] - Staining Gp83 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ADAM7 Antibody (4D1) [NBP2-75396] - Analysis of paraffin-embedded rat epididymis tissue using anti-Gp83 antibody. Counter stained with hematoxylin.

Product Discontinued

ADAM7 Antibody (4D1) Summary

• Immunogen: Recombinant protein with Human ADAM7 aa 215-315 / 824. (SwissProt: Q9H2U9 Human; SwissProt: O35227 Mouse; SwissProt: Q63180 Rat)
• Localization: Membrane.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for ADAM7 Antibody (4D1)

• a disintegrin and metalloproteinase domain 7
• ADAM 7
• ADAM metallopeptidase domain 7
• disintegrin and metalloproteinase domain-containing protein 7
• EAPI
• epididymal apical protein I
• GP83
• GP-83
• Sperm maturation-related glycoprotein GP-83

Background

The ADAM family is composed of zinc-binding proteins that can function as adhesion proteins and/or endopeptidases. They are involved in a number of biologic processes, including fertilization, neurogenesis, muscle development, and immune response.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAM7